The HSQC (Heteronuclear Single Quantum Coherence) experiment is used to determine proton-carbon single bond correlations, where the protons lie along the observed F2 (X) axis and the carbons are along the F1 (Y) axis. An edited HSQC spectrum of …

The HSQC experiment can be performed using the natural abundance of the 15 N isotope, but normally for protein NMR, isotopically labeled proteins are used. Such labelled proteins are usually produced by expressing the protein in cells grown in 15 N-labelled media.

HSQC - Heteronuclear Multiple Quantum Correlation. This 2D experiment correlates the chemical shift of proton with the chemical shift of the directly bonded carbon. On the bottom axis is a proton spectrum and on the other is a carbon. Cross peaks give …

The HSQC pulse sequence is given below: (a) The INEPT sequence (part A +B) creates S-spin antiphase coherence with respect to spin I and the S-spin coherence is frequency labeled during t1 (period C).

Heteronuclear Single Quantum Coherence (HSQC) is a technique used in NMR spectroscopy to simultaneously record 1H and 13C chemical shifts by coupling 1H nuclei with other NMR-active nuclei directly bonded to them, providing structural information in complex mixtures. You might find these chapters and articles relevant to this topic.

该谱图表明,对氨基苯甲酸乙酷分子中有五种不等性的氢 (没有考虑芳环上氢的区别)。 根据1H 的化学位移可初步确定：σ为1.3401~1.3758 的三重峰是甲考峰：4.0779的宽峰是氨上的两个氢，峰形表明这两个氢是活泼氢;4.2865-4.3399的四重峰是亚甲基峰-5.5152--6. 6505.及7.8384--7,8727 的多重峰是苯环上四个氢的峰。 2：13C-NMR谱. 碳谱能镦感地反映碳核所处化学环境的细微差别。 该谱图表明：对氨基苯甲酸乙酯分子中有七种不等性的碳核。

2008年9月1日 · 对CH相关谱而言, 单量子和多量子大的驰豫速率差别就不明显, 所以HMQC一般用于测定CH相关谱. HMQC的优点是压水峰简单, 同样记谱时间信噪比高于HSQC. HSQC (异核单量子相干谱)的优点反式检测氢维 (f2)分辨率高，灵敏度高。 缺点碳维 (f1)分辨率低。

在核磁共振（NMR）技术中，HSQC（Heteronuclear Single Quantum Coherence，异核单量子相关谱）和HMBC（Heteronuclear Multiple Bond Correlation，异核多键相关谱）是两种常用的二维谱图方法，它们主要用于确定有机分子中碳原子和氢原子之间的关联，从而帮助解析分子结构。

The 3D NOESY-HSQC experiment is specifically designed to obtain X-edited NOESY spectra of X-labeled biomolecules and protein-ligand complexes from which homonuclear 1 H- 1 H NOEs can be clearly assigned even in overcrowded regions (the mechanism involves 1 H- 1 H NOE step followed by an heteronuclear transfer via 1 J (XH)).

ABSTRACT: A vectorial representation of the full sequence of events occurring during the 2D-NMR heteronuclear single-quantum correlation (HSQC) experiment is presented.