
Heteronuclear single quantum coherence spectroscopy - Wikipedia
The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of …
利用1H-15N-HSQC二维谱确定蛋白质和配体的相互作用界面
1H-15N HSQC原理介绍:1H-15N HSQC谱,全称为1H-15N 异核单量子相干谱(1H-15N Heteronuclear Single Quantum Coherence spectroscopy),是生物核磁研究中最基础也是最 …
有机化合物核磁共振(NMR)谱图解析 - 知乎 - 知乎专栏
HSQC谱可联系相隔1个共价键的氢核和碳核,如-CH-。 如下图,当知道某氢核的谱峰位置,顺藤摸瓜,通过QC谱图,找到相对应的碳核信号。 如遇像-CH2-这两个氢非化学等价的情况,那 …
Protein Labeling and Structure Determination by NMR Spectroscopy …
2021年8月26日 · Depending on the pH and temperature of the sample, peaks may be observed from the side-chains NH groups of Arg, His, and Lys. Specifically tailored [15 N-1 H] HSQC for …
原 】 有机基础知识---二维核磁谱(2D-NMR) - 360doc
2024年5月22日 · HSQC :异核单量子关系 (heteronuclear singular quantum correlation , HSQC), 隶属于 NMR (核磁共振) C-H COSY 谱图中的一种。 HSQC 与 HMQC 的谱图相 …
2D HSQC - NMR Wiki
2010年7月2日 · 2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example 1 H, 15 …
Solution Structure and NMR Chemical Shift Perturbations of the ...
2 天之前 · The sidechain assignments were achieved using 3D-C (CO)NH, H (CCO)NH, and HBHA (CO)NH and further by using [13 C, 1 H] HSQC and HCCH-TOCSY. CS-Rosetta was …
UPLC-Q-TOF–MS/MS and NMR studies for the structural
2025年3月14日 · Both -NH and -NH 2 are assigned using 15 N-HSQC spectrum (Fig. S4 a and b). Contour peaks positioned at δ 125.6, 7.446 ppm and 110.9, 6.779 ppm chemical shifts (15 N, …
多糖结构解析之核磁二维HSQC谱图 - 知乎 - 知乎专栏
多糖的结构解析中,hsqc二维核磁是开展糖苷键连接确认的基础谱图。从有机物的hsqc解谱而言,代表的相连接的c-h相关,比如乙醇ch3-ch2-oh,其hsqc谱图中,甲基的质子和甲基碳有相 …
Protein Studies by High Pressure NMR | SpringerLink
2025年3月15日 · 1 H/ 15 N HSQC spectra of apo-myoglobin at various pressures from 3 to 300 MPa at 35 °C, adopted from Fig. 1.2 of Ref. ... The filled columns are for the 28 NH protons …