HSQC-TOCSY solves a major problem with the proton-proton version of TOCSY, which is overlap from the many cross peaks. TOCSY cross-peaks are resolved into the 13 C dimension and allows much easier assignment.

The 3D HSQC-TOCSY experiment is specifically designed to obtain X-edited TOCSY spectra from which 1 H-1 H TOCSY correlations can be clearly assigned even in overcrowded regions (the mechanism involves an heteronuclear transfer …

The 1H-13C HSQC-TOCSY experiment combines 1H-13C HSQC with a 1H-TOCSY experiments to give through-bond correlations between a 13C-attached 1H to all other coupled 1H. The coupled 1H's can be seen along a line at the same 13C chemical shift from the carbon atom attached to the primary 1H.

COSY experiments provide information about 1H-1H coupling, showing crosspeaks for proton pairs having direct JHH couplings ranging 2.5 to 25 Hz for standard COSY and DQ-COSY, and smaller JHH using (long-range) lr-COSY. TOCSY1 is the last variant of homonuclear 2D experiments that we’ll investigate. The standard COSY is shown below next to TOCSY:

2025年3月24日 · An HSQC-TOCSY experiment stands for Heteronuclear Single Quantum Coherence-Total Correlation Spectroscopy and other variants include HMQC-TOCSY, HSQC-COSY, etc. Depending on the mixing time, the hybrid experiment generally offers information on both short-range and long-range coupled nuclei.

HSQC - Heteronuclear Multiple Quantum Correlation. This 2D experiment correlates the chemical shift of proton with the chemical shift of the directly bonded carbon.

2005年9月1日 · We propose modifications of the sensitivity-improved gradient-enhanced 2D HSQC–TOCSY pulse scheme that yields a set of different selective spin-state cross-peak pattern correlation spectra with optimal sensitivity and without extra experimental setup requirements than the original decoupled experiment.

2018年3月2日 · We have developed a systematic approach for prioritization of microbial isolates for natural product discovery based on heteronuclear single-quantum correlation–total correlation spectroscopy (HSQC–TOCSY) nuclear magnetic resonance profiles in combination with antiplasmodial activity of extracts.

2022年10月1日 · New three-module NORD experiments deliver four spectra, including HMBC, HSQC, TOCSY, and either HSQC-TOCSY or H2BC. A homonuclear TOCSY module acts as recovery delay for the heteronuclear modules. Double editing according to odd/even 13 C multiplicity and one-bond/long-range correlation.

HSQC-TOCSY experiments are useful for determining structures of unknown compounds via multiple bond correlations. By varying mixing time, it is possible to tune these experiments to short and long heteronuclear correlations.