Transforming growth factor beta (TGF beta) regulates the growth of human umbilical vein endothelial cells (HUVEC) differently depending on the isoform of TGF beta and the culture conditions. The cells are resistant to growth inhibition by TGF beta when the cells are cultured on substratum coated wit …

2017年4月4日 · Proteins and mRNAs associated with EndMT were examined in human umbilical vein endothelial cells (HUVECs) treated with transforming growth factor-beta1 (TGF-β1) at different doses or at different intervals with western blotting, qRT-PCR and ELISA assays. Cell motility and migration were evaluated with motility and migration assays.

Increased extracellular TG2 or TGF-β 1 induced p-Smad2/3 signaling and endothelial-mesenchymal transition, demonstrated by the loss of CD31 and VE-cadherin expression and increased levels of ...

本研究拟通过建立转化生长因子-β (transforming growth factor-β, TGF-β)诱导人脐静脉内皮细胞 (human umbilical vein endothelial cell, HUVEC)EndMT模型，观察ACP抑制EndMT的作用及相关机制。 HUVEC购自ATCC (ATCC-CRL-1730 TM)。 黄花乌头药材 (批号：150910)，购于上海康桥中药饮片有限公司；ACP为本课题组自制。

目的：研究血根碱（SAN）对TGF?β1诱导人脐静脉内皮细胞（HUVEC）向间质细胞转化的影响，并探讨其发生机制。 方法使用TGF?β1（10μg/L）刺激HUVEC，观察不同浓度SAN对TGF?β1诱导HUVEC向间质细胞转化的影响。

... evaluate the signalling pathways involved in TGF-b1-induced EndMT in HUVECs, we examined phosphorylation of factors involved in the canonical and non-canonical signalling pathways by western...

2005年4月8日 · Current results indicate that the activation of TGF- β accompanies the apoptosis of HUVECs, and may play a protective feedback role against apoptotic cell death. The results suggest a role for...

Results: High glucose induced EndMT and increased the TGF-β1 level in HUVEC cells. Cells in high glucose for 7 days showed a significant decrease in mRNA expression of CD31 and VE-cadherin, and a significant increase in that of α-SMA and collagen I, while lost CD31 staining and acquired α-SMA staining.

As presented in Fig. 1A, the HUVEcs were treated with 10 ng/ml TGF-β1 for various durations, and the VE-cadherin and α-SMA levels were most significantly changed at 72 h, thus treatment with 10...

1995年5月25日 · Transforming growth factor beta 1 (TGF-beta 1) is a potent growth inhibitor of human umbilical vein endothelial cells (HUVEC). We investigated whether apoptosis is involved in the TGF-beta 1 mediated HUVEC growth inhibition. Using immunofluorescence microscopy and staining by propidium iodide, we sh …