Activity of the Hepatitis A Virus IRES Requires Association between …
2001年1月16日 · The question of whether translation initiation factor eIF4E and the complete eIF4G polypeptide are required for initiation dependent on the IRES (internal ribosome entry …
A conserved RNA structural motif for organizing topology within ...
2019年8月9日 · Moreover, a recent biochemical and biophysical study suggested that polio virus (PV) and HAV IRES domains may share a common mechanism of binding and utilizing …
Hepatitis A virus internal ribosome entry site (IRES) - Wikipedia
Cleavage of eIF4G, a protein scaffold of eIF4F, by sequence specific proteases 2A protease or L-protease, will result in highly inhibited HAV IRES activity. These proteases are encoded by …
Detailed Analysis of the Requirements of Hepatitis A Virus Internal ...
The HAV IRES can bind both intact eIF4G and the proteolytic C-terminal fragment with similar affinities. The C-terminal cleavage product of eIF4G with its associated eIF4A and eIF3 (Cp c …
Activity of the hepatitis A virus IRES requires association ... - PubMed
The question of whether translation initiation factor eIF4E and the complete eIF4G polypeptide are required for initiation dependent on the IRES (internal ribosome entry site) of hepatitis A virus …
Intact Eukaryotic Initiation Factor 4G Is Required for Hepatitis A ...
1997年10月13日 · Translation inhibition correlated closely with the extent of cleavage of the one identified common cellular target for the 2A and Lb proteinases, eukaryotic initiation factor …
Cellular cap-binding protein, eIF4E, promotes picornavirus …
2017年9月5日 · Here we demonstrate that eIF4E regulates HAV IRES-mediated translation by two distinct mechanisms. First, eIF4E binding to eIF4G generates a high-affinity binding …
Detailed analysis of the requirements of hepatitis A virus internal ...
The hepatitis A virus (HAV) internal ribosome entry segment (IRES) is unique among the picornavirus IRESs in that it is inactive in the presence of either the entero- and rhinovirus 2A …
Translation of Hepatitis A Virus IRES Is Upregulated by a Hepatic …
These results suggest that HAV IRES-mediated translation can be stimulated by a poly(A) tail in Huh-7 cell as well as in HeLa cell extracts. The poly(A) tail interacts with PABP, which …
Translation Directed by Hepatitis A Virus IRES in the Absence of …
2012年12月18日 · Our present findings clearly demonstrate that protein synthesis directed by HAV IRES can occur when eIF4G has been cleaved and after inactivation of eIF2. Therefore, …