Kinetic profiles of photocurrents in cells expressing two types …
2018年2月12日 · In HEK293 cells stably expressing ChR2, photocurrents were elicited at stimuli of 400–550 nm, and the wavelength sensitivity range was expanded by the additional …
Computational Optogenetics: Empirically-Derived Voltage- and
2013年9月12日 · Focusing on one of the most widely used ChR2 mutants (H134R) with enhanced current, we collected a comprehensive experimental data set of the response of this ion …
Machine learning-guided channelrhodopsin engineering enables ... - Nature
2019年10月14日 · ChRger2 enables light-induced neuronal excitation without fiber-optic implantation; that is, this opsin enables transcranial optogenetics. ChRs are light-gated ion …
Characterization of the tail current of Channelrhodopsin-2 variants
2024年9月1日 · We demonstrated that the DC pair mutations, including D156H, D156C, and C128T, are attributed to the tail current. The tail current of D156H-T159S ChR2 is related to …
Scanless two-photon excitation of channelrhodopsin-2 - Nature
2010年9月19日 · We used whole-cell patch-clamp recordings from HEK 293 cells transfected with a plasmid encoding GFP-tagged mutant of ChR2 (ChR2(H134R)-GFP) to measure ionic …
Multidimensional screening yields channelrhodopsin variants …
2019年3月15日 · We developed an automated, time-resolved screening method capable of rapidly phenotyping channelrhodopsin-2 (ChR2) variants. We found substitution mutations …
In-Depth Activation of Channelrhodopsin 2-Sensitized Excitable …
2008年10月15日 · ChR2 expression was identified by YFP imaging of HEK cells not incubated with ATR (Fig. 1 a) using a 514 nm Ar-ion laser beam. Changes in intracellular Ca 2+ levels …
Characterization of Engineered Channelrhodopsin Variants …
2009年3月4日 · Channelrhodopsin 2 (ChR2), a light-activated nonselective cationic channel from Chlamydomonas reinhardtii, has become a useful tool to excite neurons into which it is …
Visual Responses of Photoreceptor-Degenerated Rats Expressing …
2017年1月23日 · To investigate the effect on visual function of dual expression of genes with different wavelength sensitivities, we transduced a modified Volvox -derived channelrhodopsin …
Channelrhodopsin-2–XXL, a powerful optogenetic tool for low …
2014年9月8日 · Channelrhodopsin-2 (ChR2) is a microbial-type rhodopsin that can be genetically expressed to depolarize neurons with light. Thereby, this “optogenetic tool” delivers cellular …