自噬标志物—LC3检测要点 - 知乎 - 知乎专栏
氯喹可以通过抑制溶酶体功能（比如升高它的pH值），抑制LC3-II的降解；巴佛洛霉素A1则是一种强效V-ATPase抑制剂，阻断溶酶体依赖的降解。 在“堵漏”的前提下，根据一段时间内LC3-II …
FKBP8 recruits LC3A to mediate Parkin‐independent mitophagy
2017年4月5日 · FKBP 8 (also called FKBP 38) is a novel mitophagy receptor that selectively recruits LC 3A to damaged mitochondria to mediate Parkin‐independent degradation of …
ProteomeXchange Dataset pxd026874
2023年11月14日 · Activity-based protein profiling (ABPP) based evaluations reveal that a derivative molecule DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to …
LC3/GABARAPs drive ubiquitin-independent recruitment of
2019年1月24日 · Atg8 localization was assessed using HA-tagged variants of all six mammalian Atg8s (LC3A, LC3B, LC3C, GBRP, GBRPL1 and GBRPL2), to avoid the functional impairment …
FKBP8 recruits LC3A to mediate Parkin-independent mitophagy
In a yeast two-hybrid screen, we identified FKBP8 as an ATG8-interacting protein. Here, we map an N-terminal LC3-interacting region (LIR) motif in FKBP8 that binds strongly to LC3A both in …
Molecular cloning and characterization of rat LC3A and …
2006年1月6日 · In present work, we cloned LC3A and LC3B, two novel variants of rat LC3. The expression patterns of LC3, LC3A, and LC3B were different in 12 normal rat adult tissues. …
Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct …
In the current study we examine, after extensively validating antibody specificity, the expression in parallel of the three LC3A, B and C proteins in human cancer cell lines, showing distinct …
LC3 和自噬关系及LC3检测相关问题 - 百度文库
Novus 提供即用型 HeLa 氯喹处理/未处理细胞裂解物 ( NBP2-49689 ) 和 Neuro2a 氯喹处理/未处理细胞裂解物 ( NBP2-49688 )，它们是 LC3 WB 检测的强烈推荐的阳 性对照。
ABPP-LC/MS results of covalent inhibitor targeting LC3A/B in HeLa …
Activity-based protein profiling (ABPP) based evaluations reveal that a derivative molecule DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells.
盘下这 7 点，自噬研究中检测 LC3 岂会那么难？！ - 知乎
图 4：使用经过氯喹（CQ）处理的 Hela 细胞蛋白裂解物（LC3 Control Cell Extracts #11972，为阳性对照），LC3A/B 抗体（12741#）进行检测