2017年12月13日 · Equipment Design and Selection: Requirements for Single-Use and Stainless Steel Systems: With the added footprint, the HETP/chromatography skid design was provided with sufficient inlets for both bags and connections to fixed vessels. The design offered capacity for 6 × 500-L disposable bags and accommodated flow rates up to 40 L/min (LPM ...

In packed columns, mass transfer efficiency is related to intimate contact and rate transfer between liquid and vapor phases. The most used concept to evaluate the height of a packed column, which is related to separation efficiency, is the HETP (Height Equivalent to Theoretical Plate), defined by the following equation:

For evaluating the column performance after packing, a column qualification test, often called "HETP and Asymetry test", is usually performed. This test consists of evaluating the distortion of a marker pulse injected after its residence time through the …

Schematic overview of the pulse test with calculations and definitions. Peak broadening is typically described as plate number N or as height equivalent to a theoretical plate (HETP). This concept is equivalent to a tanks-in-series model reflecting the number of equilibrium stages represented by the column.

The evaluation of a column’s packing efficiency (HETP and peak asymmetry) can provide valuable knowledge about a column’s performance and stability during a purification run. The HETP (height equivalent of a theoretical plate) and asymmetry values are obtained using the protocol described below.

Each Bio-Rad chromatography skid will perform over a predefined range of column diameters and process flow rates. It is easy to select the appropriate skid category from the tables (shown at right) for gradient or isocratic systems. The application ranges of the skid categories overlap, and therefore it is possible that one

2023年12月18日 · 最常用的柱效测定方法，即产生一个狭窄的样品峰，依次评估峰增宽和峰的对称性。这两个指标分别称为 HETP （Height equivalent to a Theoretical Plate）和As( Asymmetry factor)。 传统上，氯化钠，苯甲醇或者丙酮被用来测试柱效。氯化钠用电导监测，而后两者使 …

The VERDOT Ips2 process chromatography skid is self-contained and incorporates all the necessary components (pumps, valves, and instruments), control cabinets (pneumatic and electric), and the touch screen interface used to run the chromatography process. The components of the skid are housed within a protective frame.

2023年10月10日 · HETP, H = L∕N. 在HPLC中，控制H的主要因素是用作填充材料的颗粒直径 (dp)。 对于填充良好的色谱柱，H大约等于2dp。 填充有5-微米材料的典型的150毫米长的柱应该具有N = L/H =150 000微米/ (2 × 5微米)，或者大约 15 000个盘子。 类似地，填充有3-微米材料的150mm柱应该具有N = L/H =150 000微米/6微米，或者大约25 000个塔板。 因此，填充较小颗粒的色谱柱效率更高，单位长度塔板数更高。 Ref.: HPLC AND UHPLC FOR PRACTICING …

2024年8月23日 · 琼脂糖基质的层析柱一般要求hetp （理论塔板高度）的数值小于5倍介质平均颗粒大小，且非对称因子在 0.8~1.8 之间（越接近于1越好），则说明装柱效果较好。