The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR. The experiment was first described by Geoffrey Bodenhausen and D. J. Ruben in 1980. [1]

Oct 24, 2012 · Magnetization is transferred from hydrogen to attached 15 N nuclei via the J-coupling. The chemical shift is evolved on the nitrogen and the magnetisation is then transferred back to the hydrogen for detection. This is the most standard experiment and shows all …

1H-15N HSQC原理介绍：1H-15N HSQC谱，全称为1H-15N 异核单量子相干谱（1H-15N Heteronuclear Single Quantum Coherence spectroscopy），是生物核磁研究中最基础也是最常用的一个二维谱。图四所示是1H-15N HSQC核磁脉冲序列：

This Chapter introduces 2D NMR spectroscopy, which greatly enhances the resolving power of the spectra in comparison with generic 1D data. Specifically, 2D ?????N-HSQC NMR spectra will be discussed …

May 22, 2024 · HSQC ：异核单量子关系 (heteronuclear singular quantum correlation ， HSQC), 隶属于 NMR （核磁共振） C-H COSY 谱图中的一种。 HSQC 与 HMQC 的谱图相同，都是显示 1 H 核和与其直接相连的 13 C 核的相关峰，其作用相应于 C,H-COSY 谱。

TOCSY skewers from the TOCSY–HSQC are used to identify spin system types and to account for intraresidue NOEs in the NOESY–HSQC. Sequential NOEs can then be identified from the latter spectrum and used to deduce interresidue connectivities as usual.

2D 1H-13C HSQC (Heteronuclear Single Quantum Coherence), indirect TD fixed at 128 increments; multiplicity-edited so CH and CH3 peaks are positive and CH2 are negative (as in a DEPT135 spectrum), sensitivity improved over standard. Gives very good signal levels on samples less than 10 mg.

Feb 2, 2017 · 摘要: 尽可能完全、准确地归属蛋白质分子的核磁共振（Nuclear Magnetic Resonance, NMR）谱峰，是解析可信赖、高质量的蛋白质三维空间溶液结构的首要条件.自动归属软件的开发和应用，已经方便并加快了蛋白质分子核磁共振谱峰的归属进程.然而，对蛋白质核磁共振研究领域的新手来说，因为缺乏对蛋白质分子的核磁共振谱峰特性的系统认识而可能发生对自动归属结果的错误指认或指认不完全，从而导致蛋白质结构解析的错误或偏差.该文针对蛋白质 …

Mar 1, 2024 · The 1 H-15 N HSQC spectrum of α-synuclein is characterized by a low cross-peak dispersion and presents many peaks in overlap one to the other, a common feature of intrinsically disordered protein spectra. The 1 H-15 N HSQC spectrum of ubiquitin shows the large cross-peak dispersion typical of folded proteins.

Oct 28, 2020 · HSQC：异核单量子关系 (heteronuclear singular quantum correlation，HSQC), 隶属于NMR（核磁共振） C-H COSY 谱图中的一种，此外还有多键碳氢关系谱 HMBC 及异核多量子关系 HMQC。 HSQC给出的信息是直接相连的碳氢关系，给出一键CH连接问题，而不能解决碳与季碳相连的问题，或隔碳相连的问题。 HMQC：异核多量子关系. HMQC和HSQC,这两种图谱的作用与H-CCOSY相当，由于是反向实验，灵敏度高。 二者相比HSQC优点更多一些。 当样品量 …