Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase ...
2016年1月20日 · The first structure of fully active HOIP of the RBR family of RING-type E3 ligases in its transfer complex with an E2~ubiquitin conjugate provides insights into its mechanism of …
The unifying catalytic mechanism of the RING-between-RING E3
2023年1月11日 · Addition of fluorescently labelled Ub (TAMRA-Ub) with a native N-terminus to GPG-Ub-loaded HOIP RBR induces the RBR aminolysis reaction, E3-Ub discharge, and …
The E3 ligase HOIP specifies linear ubiquitin chain assembly …
Here, we show that both LUBAC catalytic activity and LUBAC specificity for linear ubiquitin chain formation are embedded within the RING‐IBR‐RING (RBR) ubiquitin ligase subunit HOIP. …
Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase ...
2016年1月28日 · HOIP RBR binds the E2~ubiquitin conjugate in an elongated fashion, with the E2 and E3 catalytic centres ideally aligned for ubiquitin transfer, which structurally both …
Structural Insights into SHARPIN-Mediated Activation of HOIP for …
2017年10月3日 · As the catalytic subunit of LUBAC, HOIP is a RING-between-RING (RBR) type E3 (Kirisako et al., 2006, Smit et al., 2012), containing mainly an N-terminal PNGase/UBA or …
RBR ligase–mediated ubiquitin transfer: a tale with many
2018年5月7日 · RBR ligases are an enigmatic class of E3 ubiquitin ligases that combine properties of RING and HECT-type E3s and undergo multilevel regulation through …
RBR E3 ubiquitin ligases: new structures, new insights, new …
2014年3月3日 · The best known of the RBR enzymes are parkin, which has a prominent role in the manifestation of early-onset Parkinson's disease, and HOIP (HOIL-1-interacting protein) …
Chain reactions: molecular mechanisms of RBR ubiquitin ligases
HOIP contains a ubiquitin-associated domain (UBA) N-terminal of the RBR core. UBA domains are small (∼45 amino acid) three-helix bundles that typically bind ubiquitin via the canonical …
Fragment-Based Covalent Ligand Screening Enables Rapid …
2019年2月2日 · We report the synthesis of a diverse library of electrophilic fragments and demonstrate an integrated use of protein LC–MS, biochemical ubiquitination assays, chemical …
Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase ...
2016年1月1日 · Here we present the first structure of the fully active HOIP-RBR in its transfer complex with an E2~ubiquitin conjugate, which elucidates the intricate nature of RBR E3 …