2025年3月21日 · Here, we present a protocol for staining the vacuole lumen in Arabidopsis root cells, enabling precise visualization of vacuolar dynamics. We describe steps for preparing plant material and staining with commonly used fluorescent dyes.

2016年4月14日 · Using a combination of cryo-soft X-ray tomography and spectroscopy, and cryo-focused ion beam scanning electron microscopy (FIB-SEM), we visualized and characterized a highly concentrated,...

A vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga

2024年1月24日 · Our findings provide evidence of the influence of Dark-vacuole bodies on the morphology, distribution, movement, and cell cycle of living cells. Specifically, we analyzed the effects of drug induced stimulation of lipid drops and endosomes, promotion of cell endocytosis, and induction of cellular senescence on Dark-vacuole bodies.

2024年12月20日 · We present a protocol for overexpressing, purifying, and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes and describe steps to measure transport activity using radioactive substrates. The protocols established here can be used to study other vacuolar or lysosomal membrane transporters.

2025年3月5日 · SOS2 phosphorylates FREE1, leading to its degradation and affecting MVB maturation, thereby reducing MVB-vacuole fusion and regulating endomembrane dynamics in response to salt stress. These findings highlight the adaptive role of the plant endomembrane system in coping with salt stress.

Vesicles and vacuoles are membrane-bound sacs that function in storage and transport. Vacuoles are somewhat larger than vesicles, and the membrane of a vacuole does not fuse with the membranes of other cellular components.

2019年3月25日 · Food vacuoles are membrane-bound sacs within a cell, which contain food matter to be digested. These can be thought of as intracellular “stomachs,” where food is stored while it is broken down and its nutrients are extracted. To begin the cellular “eating” process, the cell membrane curves to envelope a particle of food.

The food vacuole, or digestive vacuole, is an organelle found in simple eukaryotes such as protists. This organelle is essentially a lysosome . During the stage of the symbiont parasites' lifecycle where it resides within a human (or other mammalian) red blood cell, it is the site of haemoglobin digestion and the formation of the large ...

2024年4月8日 · The food vacuole, a specialized cellular compartment, is characterized by its adaptability in structure, tailored to meet the specific needs and demands of the cell it resides in. Here, we delve into the intricate structural aspects of the food vacuole: Morphological Variability: The food vacuole does not adhere to a fixed size or shape.