We show here that in human cells the overexpression of green fluorescent protein-tagged heterochromatin protein 1 (GFP-HP1) or nontagged HP1 isoforms HP1 Hsα or HP1 Hsβ, but not HP1 Hsγ, results in decreased association of a catalytic …

Plasmid GFP-HP1alpha from Dr. Tom Misteli's lab contains the insert HP1 alpha and is published in Science. 2003 Jan 31. 299 (5607):721-5. This plasmid is available through Addgene.

2021年1月22日 · We show that an antiGFP nanobody can be used to simultaneously visualize GFP-tagged chromatin regulators and control gene expression, and that nanobodies against HP1 and DNMT1 can silence a...

2005年10月12日 · Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1) to discrete regions of the genome, thereby regulating gene expression, chromatin packaging and...

2020年4月16日 · HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanistically relevant features of pericentric heterochromatin compaction in mouse fibroblasts.

2018年3月16日 · In this study, we identified Plasmodium falciparum gametocyte development 1 (GDV1) as an upstream activator of sexual commitment. We found that GDV1 targeted heterochromatin and triggered HP1 eviction, thus derepressing ap2-g. Expression of GDV1 was responsive to environmental triggers of sexual conversion and controlled via a …

2023年11月28日 · Our results demonstrate the role of HP1 proteins in regulating histone modification crosstalk during establishment and identify a genetically separable function in maintaining epigenetic memory. Chromatin organization is critical for maintaining genome stability and regulating gene expression.

2018年2月2日 · In this article, Sridharan and colleagues perform proteomic characterization of the heterochromatin protein 1 (HP1) family in reprogramming and pluripotency. Depletion of HP1γ or its interacting partner SENP7, which anchors it to heterochromatin, increased iPSC generation.

2022年12月19日 · Hp1α/β deficiency reduces cell proliferation, ribosomal RNA biosynthesis and mobility of Nucleophosmin, a major nucleolar component. Nucleolar integrity and function require HP1α/β proteins to be recruited to H3K9me3-marked PCH and their ability to dimerize.

2017年6月21日 · We show that Drosophila HP1a protein undergoes liquid–liquid demixing in vitro, and nucleates into foci that display liquid properties during the first stages of heterochromatin domain formation in...

Our research demonstrated that HP1α, HP1β, and HP1γ facilitate the positioning of ATRX within the mitotic centromere area through their interaction with the first two [P/L]-X-V-X- [M/L/V] (PxVxL)motifs at the N-terminus of ATRX. ATRX deficiency causes aberrant mitosis and decreased centromeric cohesion.

2015年9月28日 · Here we identify Heterochromatin Protein 1β (HP1β) as an essential protein for proper differentiation, and, unexpectedly, for the maintenance of pluripotency in ESCs. In pluripotent and differentiated cells HP1β is differentially localized …

2007年10月1日 · We have compared the distribution of endogenous heterochromatin protein 1 (HP1) proteins (alpha, beta and gamma) in different epithelial lines, pluripotent stem cells and embryonic fibroblasts. In parallel, we have interrogated assembly and dynamics of newly expressed HP1-GFP proteins in cells lacki …

2024年8月28日 · Specifically, we find that HP1α monomers, which are the dominant species according to fluorescence fluctuation spectroscopy (FFS) of HP1α tagged to eGFP, impart a global spacing between nucleosomes that is mediated by H3K9me3, and this spacer can be locally removed upon HP1α dimer formation.

1999年10月1日 · We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome assembly during DNA replication.

2008年3月16日 · To investigate how STAT92E activation by phosphorylation leads to heterochromatin destabilization, we examined STAT92E–GFP and HP1 localization in ex vivo cultured salivary glands at different...

2018年8月6日 · Anti-GFP antibody was used to detect LHP1::GFP. (B) Identification of ATRX regions that directly interact with LHP1 using the yeast two-hybrid assay. Various truncated regions of ATRX as indicated on the left and full length LHP1 were fused with the GAL4 AD and BD domain, respectively.

2018年11月13日 · Here, we report on the discovery of ADCP1, a plant-specific triple tandem Agenet protein, as a multivalent H3K9me reader in Arabidopsis, and establish that ADCP1 is essential for heterochromatin...

2022年5月7日 · HP1 proteins are highly conserved, and many eukaryotic genomes contain multiple HP1 genes. Given the presence of multiple HP1 family members within a genome, HP1 proteins can have unique as well as shared functions. Here, we review the mechanisms by which HP1 proteins contribute to the regulation of transcription.

2017年11月21日 · Analysis of Hp1α −/−, Hp1β −/−, and Hp1γ −/− MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1α confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin ...