2021年4月14日 · Our work identifies HP1a as an epigenetic regulator that is involved in establishing the global structure of the genome in the early embryo. In metazoans, fertilization triggers global de novo...

2024年8月28日 · Selective siRNA knockdown (KD) of endogenous HP1α in wild type HeLa (HeLa KD) and the cell lines exogenously transfected with eGFP-HP1α, eGFP-HP1α I165E or HP1α I165E (HeLa eGFP-HP1α+KD, HeLa eGFP-HP1αI165E+KD, and HeLa HP1αI165E+KD) was achieved via transfection of three siRNA duplexes designed to target the mRNA 3′ untranslated ...

2023年3月9日 · We find that high level of HP1γ is associated with low acetylation modification in the bortezomib-resistant myeloma cells using SILAC-based acetyl-proteomics assay, and higher HP1γ level is...

The HP1-rescue construct reverses the structural effects of HP1-KD (reduced contact frequency between the pericentromeric regions, as well as inter-chromosome arm interactions and compaction defects).

2014年3月12日 · Our results reveal that NDR kinase catalyses the hinge-specific phosphorylation of human HP1α during G2/M in vivo and this orchestrates accurate chromosome alignment and mitotic progression....

2013年5月17日 · Heterochromatin protein 1 (HP1) is an epigenetic gene silencing protein that is regulated by lysine 9 methylation of histone H3. Most eukaryotes have at least three HP1 homologs with similar domain structures but with different localization patterns and functions in heterochromatin and euchromatin.

2021年5月13日 · Here, by combining chromosome conformation capture (Hi-C), chromatin immunoprecipitation with high-throughput sequencing (ChIP–seq), 3D DNA fluorescence in situ hybridization (3D DNA FISH) and...

KD of heterochromatin factor HP1γ increases expression of unintegrated HIV-1 DNAs. (A) Transient siRNA-based KD was performed for the three HP1 isoforms: HP1α, HP1β, HP1γ. Representative...

We propose that the totipotent stem cell state was shaped by the need to resist transposon activity over evolutionary timescales. ... Therefore, dMes-4/NSD counteracts H3K27me3 spreading by...

Hi-C analysis of HP1-KD embryos at ZGA revealed major genome-wide changes in chromosome organization (Fig. 3a, Extended Data Fig. 3g, h); we found perturbed Rabl configuration with decreased contact frequencies within and between pericentromeric regions and reduced inter-arm and inter-chromosomal contacts (Fig. 3a).