HpaII is an isoschizomer of MspI. Inhibited by salt concentrations > 50 mM KCl. Blocked by CpG methylation. Why Choose Recombinant Enzymes? Is HpaII inhibited by salt? Is HpaII affected by methylation? What is the molecular weight of HpaII? Does spermidine increase HpaII activity? Does HpaII cleave ssDNA? What is the activity of HpaII at 25°C?

HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from certain region as demonstrated below. [1] It has the ability to produce cohesive ends, which are rather useful in constructing plasmids.

Thermo Scientific HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37C in Tango buffer. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one

HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C 5) of the sequence CCGG. An E. coli strain that carries the cloned HpaII modification gene from Haemophilus parainfluenzae (ATCC 49669).

5-methylcytosine is a prominent epigenetic DNA modification which plays an important role in up- and down-regulation of gene expression. The Thermo ScientificTM EpiJETTM DNA Methylation Analysis Kit (MspI/HpaII) uses a pair of restriction enzymes with different sensitivity to methylation to analyze DNA methylation status at a specific locus.

2021年1月15日 · The MSREs HpaII, Hin6I, AciI, and HpyCH4IV together cover six of the nine canonical CpG methylation sites of the FOXP3 TSDR . A demethylated cytosine located at any one of these six CpG sites allows restriction enzyme digestion by a MSRE, preventing the amplification of the TSDR region.

1980年1月1日 · This chapter discusses the preparation and properties of the HpaI and HpaII endonucleases. Two restriction endonuclease activities, HpaI and HpaII are isolated from Haemophilus parainfluenzae. Endonuclease HpaI has been isolated in homogeneous form, while HpaII has been purified free of contaminating nuclease and phosphatase activities.

The gene for the HpaII methylase has been cloned into E. coli and its nucleotide sequence has been determined. The DNA of the clones is fully protected against cleavage by the HpaII restriction enzyme in vitro, indicating that the methylase gene is active in E. coli.

HpaII-amplifiable fragments are defined as those that can be (a) amplified using our LM-PCR approach and (b) into which we can place an oligonucleotide for microarray interrogation, giving us a lower limit of 50 bp in size.

The secondary digestion of these fragments with methylation-sensitive enzymes, BstUI or HpaII, helps remove umethylated fragments. The methylated products are first amplified by primers that anneal to the linker sequence, then fluorescently labeled, and finally hybridized to arrays ( 68 ).