发夹干扰载体 - BIORUN
在RNAi研究中，通常通过向细胞内导入发夹RNA（hairpin RNA， hpRNA）来引起干扰，即在构建植物转基因的双元载体时，通过强启动子驱动一个“正向序列一环一互补序列”的转录单元进行 …
Nucleotide mismatches prevent intrinsic self-silencing of hpRNA ...
2022年7月7日 · Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method in plants. Here, we show that hpRNA transgenes are invariably methylated in …
植物hprna干扰载体构建的研究进展 - 豆丁网
2016年4月26日 · 摘要：RNAi技术在基因功能鉴定和功能基因表达调控中发挥着重要作用。 hpRNA干扰载体被广泛用于植物的RNAi研究。 对植物hpRNA干扰载体的构建方法进行综述， …
TaqTth-hpRNA: a novel compact RNA-targeting tool for specific …
2024年7月7日 · Pathogenic allele silencing is a promising treatment for genetic hereditary diseases. Here, we develop an RNA-cleaving tool, TaqTth-hpRNA, consisting of a small, …
5’ triphosphate hairpin RNA - RIG-I agonist - InvivoGen
3p-hpRNA (tlrl-hprna) is a specific RIG-I agonist generated by in vitro transcription of an influenza A virus sequence. This triphosphate dsRNA (3pRNA) is endotoxin-free and its potency is …
Full-Length Hairpin RNA Accumulates at High Levels in Yeast ... - PubMed
2019年6月15日 · Hairpin-structured (hp) RNA has been widely used to induce RNA interference (RNAi) in plants and animals, and an in vivo expression system for hpRNA is important for …
RNA Silencing in Plants: Mechanisms, Technologies and …
RNA silencing is an evolutionarily conserved mechanism in eukaryotes. It is induced by double-stranded RNA (dsRNA) or hairpin structured RNA (hpRNA), involving common factors …
Chemical induction of hairpin RNAi molecules to silence vital …
2016年11月29日 · Understanding the functions encoded by plant genes can be facilitated by reducing transcript levels by hairpin RNA (hpRNA) mediated silencing. A bottleneck to this …
Nucleotide mismatches prevent intrinsic self-silencing of hpRNA ...
2022年7月7日 · Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method in plants. Here, we show that hpRNA transgenes are invariably methylated in …
RNA介导的基因沉默 - RNA 介导的基因沉默实验 -丁香实验
2024年5月13日 · 发夹 RNA 载体（hpRNA ) 的产生 构建 hpRNA 的方法有多种。 可以利用标准的植物转化载体进行构建，这种方法需要将每个目的基因对应的发夹编码结构都重新构建到载体 …