2009年11月13日 · In our crystal structure, three specific metal binding sites (Ca1–3) were identified and these metal ions are possibly involved in the C-terminal cleavage of HycE. Hydrogenases catalyze the reversible formation of molecular hydrogen (2H + + 2e − ↔ H 2), and thus contribute to energy metabolism in a variety of microorganisms.

1998年2月11日 · Maturation of the large subunit of E. coli hydrogenase 3, HycE, requires the action of seven accessory proteins. The HycI protease catalyses a C-terminal proteolytic cleavage of the large subunit, which was shown to result in a dramatic change in migration behavior of HycE in nondenaturing PAGE.

2010年12月29日 · 构建了hycE基因缺失突变体ΔhycE，用于阐明hyc-操纵子在氢代谢中的功能。 与野生型菌株HP1相比，该突变菌株在O（2）胁迫条件下在甲酸，丙酮酸钠和葡萄糖存在下显示出氢气产生显着减少，而在厌氧条件下检测到大量活性。

2000年5月12日 · The steps in the maturation of the precursor of the large subunit (pre-HycE) of hydrogenase 3 from Escherichia coli taking place after incorporation of both iron and nickel were investigated. Pre-HycE could be matured and processed in the absence of the small subunit but association with the cytoplasmic membrane required heterodimer formation ...

产气肠杆菌的[NiFe]-氢化酶3包含一个60 kDa的大亚基（HycE）和一个30 k Da的小亚基（HycG）。 使用pGEX-4T-2-cat载体分别在产气大肠杆菌中过表达HycE基因和HycG基因，得到两个重组菌株：产气大肠杆菌/ HycE和产气大肠杆菌/ HycG。

2011年12月16日 · Although the isolated proteins have been characterized, little is known about how they interact with each other and the hydrogenase 3 large subunit, HycE. In this study the complexes of HypA and HycE were investigated after modification with the Strep-tag II.

HycI is an endopeptidase of the M52 family and responsible for the C-terminal cleavage of the large subunit of hydrogenase 3 in Escherichia coli. Although extensive studies were performed, the molecular mechanism of recognition and cleavage of hydrogenase 3 remains elusive.

1998年3月3日 · Maturation of the large subunit of E. coli hydrogenase 3, HycE, requires the action of seven accessory proteins. The HycI protease catalyses a C-terminal proteolytic cleavage of the large subunit, which was shown to result in a dramatic change in migration behavior of HycE in nondenaturing PAGE.

2008年5月1日 · The large subunit (HycE, 569 amino acids) of Escherichia coli hydrogenase 3 produces hydrogen from formate via its Ni–Fe-binding site. In this paper, we engineered HycE for enhanced hydrogen production by an error-prone polymerase chain reaction (epPCR) using a host that lacked hydrogenase activity via the hyaB hybC hycE mutations.

2009年11月1日 · Here, we present the crystal structure of HycI at 1.70 Å resolution. The crystal structure resembles the recently reported solution structure (NMR) of the same protein and the holo-HyPD structure...