Here we describe the conditions for an efficient BrdU-labeling coupled to an immunodetection of neuronal markers, either regulators of neurogenesis, here the homeoprotein prdl-a, or …

We demonstrate that proliferation of stem cells in Hydra is robust against the disturbance of Lamin expression and localization. While Lamin is indispensable for Hydra, the stem cells tolerate …

2019年1月1日 · In this study we identified four paralogs of the Hym-176 neuropeptide gene (Yum et al., 1998a) in Hydra ESTs and found clearly regionalized peptidergic neuron subsets based …

2009年8月18日 · To characterize the apoptosis-induced proliferative response, we performed a systematic analysis of cell cycling over the first 8 hpa: Hydra were pulse labeled with BrdU for …

2019年9月25日 · Here we describe the conditions for an efficient BrdU-labeling coupled to an immunodetection of neuronal markers, either regulators of neurogenesis, here the …

2019年4月26日 · BrdU and prdl‐a Double Immuno‐Labeling Intact H. vulgaris were incubated with 5 mM BrdU for 4 h and either amputated at mid‐gastric level and let to regenerate for four …

1991年12月1日 · Epithelial animals were pulse labeled with the thymidine analog 5′-bromo-2′-deoxyuridine (Brdu) and stained with anti-Brdu antibody to visualize S phase cells. Our results …

Exposure of hydra to 3.25×10 −3 M BrdU selectively altered differentiation in the animal's pluripotent I-cell population. It is suggested, therefore, that this analog may represent a valid …

Hansen GN, Williamson M, Grimmelikhuijzen CJ (2000) Two-color double-labeling in situ hybridization of whole-mount Hydra using RNA probes for five different Hydra neuropeptide …

The present results on Brdu labeling Schaller (1976, 1977; Schaller et al., 1990) has shown that treatment of hydra with low concentrations of a neuropeptide, the head activator, stimulates …