2015年12月22日 · To confirm the existence of C2 subclusters, IB4-negative small neurons (area < 314 μm 2), selected randomly from dissociated DRG neurons, were analyzed by single-cell PCR .

1997年10月1日 · We have tested the role of glial cell line-derived neurotrophic factor (GDNF) in regulating a group of putatively nociceptive dorsal root ganglion (DRG) neurons that do not express calcitonin gene-related peptide (CGRP) and that downregulate the nerve growth factor (NGF) receptor tyrosine kinase, TrkA, after birth.

2020年6月24日 · 我们使用整装小鼠腰椎 DRG 制剂、共聚焦显微镜和计算机辅助 3D 分析来揭示 IB4 +非肽能神经元形成 4.7 ± 0.26 个细胞的小簇，不同于 CGRP +大多数情况下，肽能神经元是孤立的（1.89 ± 0.11 个细胞）。 神经元的两个亚群都被一层薄薄的卫星神经胶质细胞 (SGC) 包裹着，可以在用特定标记谷氨酰胺合成酶 (GS) 进行免疫标记后观察到。 值得注意的是，在超微结构水平上，我们观察到这个神经胶质层是不连续的，因为两个相邻 IB4 +神经元的膜之间存在直接 …

We characterized the membrane excitability of IB4 + and IB4 – subpopulations of small DRG neurons, examined the responses of IB4 + and IB4 – DRG neurons to acute oxaliplatin, and studied the effects of antagonists of membrane depolarization and subtypes of TRP channels on the membrane hyperexcitability and/or pain behaviors induced by ...

2021年11月2日 · The distribution of MS currents in DRG neurons has been addressed over the past by distinguishing neuronal subsets using morphological and electrophysiological parameters as well as IB4 staining (Coste et al., 2007; Drew et al., 2002, 2004; Hu and Lewin, 2006; Lechner and Lewin, 2009; Prato et al., 2017). These approaches led to the view that ...

2024年3月4日 · The reporter signals detected in DRG neuron cell bodies were compared with signals for three neurochemical makers: CGRP, which labels many small and large diameter “peptidergic” DRG neurons; Isolectin B4 (IB4), which labels a subset of small-diameter “nonpeptidergic” C-fiber neuron subtypes; and NEFH, which labels medium and large ...

2007年1月31日 · In cultures of dissociated DRG neurons of adult mice on laminin, robust spontaneous neurite outgrowth from IB4-negative neurons occurs and is strongly enhanced by previous axotomy. In contrast, IB4-labeled neurons show little neurite outgrowth and do not express GAP 43, even after axotomy or culture with GDNF.

2016年10月7日 · In vitro, GDNF supports the survival of small neurons that express Ret and bind IB4 while failing to support the survival of neurons expressing TrkA and CGRP. Together, our findings suggest that IB4-binding neurons switch from dependence on NGF in embryonic life to dependence on GDNF in postnatal life and are likely regulated by GDNF in maturity.

2006年7月5日 · Binding to isolectin-B4 (IB4) and expression of tyrosine kinase A (trkA) (the high-affinity NGF receptor) have been used to define two different subgroups of nociceptive small dorsal root ganglion (DRG) neurons.

2015年11月18日 · Amongst nociceptive DRG neurons, a mechanically sensitive neuron, isolectin B4 negative Aδ-type (IB4 − Aδ) DRG neuron displays spontaneous activity with hyperexcitability after chronic...