Prokaryotic Gabija complex senses and executes nucleotide …
2023年7月23日 · The Gabija complex is a prokaryotic antiviral system consisting of the GajA and GajB proteins. GajA was identified as a DNA nicking endonuclease but the functions of GajB …
Structural basis of Gabija anti-phage defence and viral immune
2023年11月22日 · Gabija protein A (GajA) is a DNA endonuclease that tetramerizes to form the core of the anti-phage defence complex. Two sets of Gabija protein B (GajB) dimers dock at …
Structures and activation mechanism of the Gabija anti-phage …
2024年3月12日 · Here, to explore how the Gabija system is activated for anti-phage defence, we report its cryo-electron microscopy structures in five states, including apo GajA, GajA in …
Structural and biochemical insights into the mechanism of the …
2024年1月29日 · GajA is a sequence-specific DNA-cleaving enzyme in the presence of Mg 2+, its activity is completely inhibited by the high concentration of NTP and dNTP 9. Prior research …
A nucleotide-sensing endonuclease from the Gabija bacterial …
2021年5月21日 · The GajA protein is revealed as a sequence-specific DNA nicking endonuclease unique in that its activity is strictly regulated by nucleotide concentration. NTP and dNTP at …
Cell子刊 | 华中科技大学首次揭示Gabija免疫机制 - 知乎
研究发现Gabija系统通过流产感染方式表现出广泛而有效的噬菌体抗性，并且GajA和GajB对其功能至关重要。GajA表现出特异性的寡聚化和DNA结合特性，后者被NTP强烈抑制。出乎意料的 …
Prokaryotic Gabija complex senses and executes nucleotide …
Cheng et al. reveal the molecular mechanism of the widespread Gabija prokaryotic immune system, in which the synergy between GajA, an NTP-sensing DNA nickase, and GajB, a DNA …
2023年8月7日 · Here, we present the cryo-EM structures of GajA alone and the GajAB complex. GajA assembles into a tetramer via interactions mediated by both the ATPase domain and the …
Molecular basis of Gabija anti-phage supramolecular assemblies
2024年4月16日 · As one of the most prevalent anti-phage defense systems in prokaryotes, Gabija consists of a Gabija protein A (GajA) and a Gabija protein B (GajB). The assembly and …
Structures and activation mechanism of the Gabija anti-phage …
Here, to explore how the Gabija system is activated for anti-phage defence, we report its cryo-electron microscopy structures in five states, including apo GajA, GajA in complex with DNA, …