HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

通过下方视频进一步了解HTRF IP-ONE Gq Kit，均相体系，操作简便，信号稳定。 https://www.zhihu.com/video/1749378802432737281. GPCR检测平台. 此外还有信号通路上游Cell-based Receptor binding assays(Tag-lite），下游Phosphorylation assays等，如果您感兴趣的话，请联系Revvity吧！ 参考文献

HTRF IP1测量试剂盒是基于HTRF技术的竞争性免疫检测，使用了铽穴状化合物（Tb cryptate）标记的抗IP1单抗和d2标记的IP1。 细胞所产生的IP1和试剂盒所提供的标记了d2的IP1竞争抗IP1抗体的抗原结合位点。 当铽标记抗IP1抗体与d2标记的IP1结合后，会发生能量共振转移，产生比较大的信号。 随着细胞内产生的IP1增多，游离的IP1与抗体结合增多，信号逐渐减小（见图3）。 HTRF技术称为均相时间分辨荧光技术（Homogeneous Time-Resolved Fluorescence）, 其结 …

HTRF IP-one系列产品是针对Gq蛋白GPCR的分析平台。 该产品通过抑制通路中IP-One的降解，来实现稳定检测Ca流通路的目的，可检测缓慢结合的GPCR。 (如图示)

ip-one 检测具有简化的方案，仅需两个孵育步骤：通过配体或目标化合物刺激细胞；使用 htrf 试剂检测 ip1。 该方案在细胞刺激后仅需一小时的单一孵育期。

HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

IP-One ELISA is a competitive immunoassay which uses IP1-HRP and an anti-IP1 monoclonal antibody. The kit comes with 96-well microplates pre-coated with an anti-mouse antibody. Cells are stimulated in the presence of LiCl, causing the accumulation of IP1 upon receptor activation.

HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

2024年12月21日 · ip-one 检测具有简化的方案，仅需两个孵育步骤：通过配体或目标化合物刺激细胞；使用 htrf 试剂检测 ip1。 该方案在细胞刺激后仅需一小时的单一孵育期。

Phospholipase C ß (PLC-ß)-coupled G protein-coupled receptor (GPCR) produce IP1, an analyte quantified by the IP-One assay. Purified IP1 can be used as a sandard to quantify the IP1 produced by cells in an experimental setup.