2018年7月16日 · IRE1α is a serine/threonine protein kinase and endoribonuclease that catalyses the unconventional processing of the messenger RNA encoding X-box binding protein 1 (XBP1), resulting in the...

2020年5月14日 · DNA damage engages IRE1α signaling in the absence of an endoplasmic reticulum (ER) stress signature, leading to the exclusive activation of regulated IRE1α-dependent decay (RIDD) without activating...

2024年5月7日 · Here we report that, in mammalian cells, formation of IRE1α clusters is an ER membrane-bound phase separation event that is coupled to the assembly of stress granules (SGs). In response to...

2015年6月23日 · To further study the role of IRE1 in inhibiting ER membrane permeabilization and cell death, we used Ire1α knockout MEFs stably expressing mammalian ER-targeted redox-sensitive GFP (MERO-GFP). MERO-GFP changes its excitation spectrum depending on the oxidation status of two engineered cysteines.

2023年11月9日 · 团队在诱导产生GFP阴性的 scrib 突变克隆的同时, 在其周围GFP阳性WT细胞中敲低或过表达Ire1/Xbp1s, 发现均可显著抑制 scrib 克隆的清除, 提高loser细胞的适应性(图3A-B), 提示可能Ire1在不同细胞中的 差异表达 是决定细胞适应性的关键。进一步团队利用果蝇的优势, 诱导 ...

2023年12月26日 · Our findings unveil a direct crosstalk between SOCE and UPR via IRE1, acting as key regulator of ER Ca 2+ and proteostasis in T cells and muscles. Under ER stress, this IRE1-STIM1 axis boosts SOCE to preserve immune cell functions, a pathway that could be targeted for cancer immunotherapy.

2024年5月8日 · 肌醇需要酶1（Inositol-requiring enzyme 1，IRE1）是位于内质网上的跨膜蛋白，通过激活其胞内段蛋白激酶和核糖核酸内切酶的双重活性，起始并控制着未折叠蛋白响应中最为保守的一条信号通路。

2022年1月5日 · Here, we provide detailed protocols to perform four different assays to study IRE1 activation and signaling: (i) Phos-tag gels to evaluate the phosphorylation status of IRE1, (ii) microscopy using TREX-IRE1-GFP cells to measure IRE1 oligomerization, (iii) conventional RT-PCR to assess XBP1 mRNA processing, and (iv) quantitative PCR to determine ...

2023年9月9日 · When misfolded proteins accumulate in the ER, the ER membrane kinase Ire1 dimerizes, autophosphorylates, and then splices the mRNA of XBP1 (HAC1 in yeast). This enables its efficient translation (Cox and Walter, 1996), giving rise to a potent transcription factor that induces the UPR ER.

2017年8月17日 · We show that the most conserved transducer of ER stress, Ire1, uses an amphipathic helix (AH) to sense membrane aberrancies and control UPR activity. In vivo and in vitro experiments, together with molecular dynamics (MD) simulations, identify the physicochemical properties of the membrane environment that control Ire1 oligomerization.