等温滴定量热法(Isothermal Titration Calorimetry, 简称ITC)是近年来发展起来的一种研究生物热力学与生物动力学的重要方法，它通过高灵敏度、高自动化的微量量热仪连续、准确地监测和记录一个变化过程的量热曲线…

In chemical thermodynamics, isothermal titration calorimetry (ITC) is a physical technique used to determine the thermodynamic parameters of interactions in solution.

Isothermal Titration Calorimetry (ITC) is a label-free method for measuring binding of any two molecules that release or absorb heat upon binding. ITC can be used to measure the thermodynamic parameters of biomolecular interactions, including affinity (K A), enthalpy (ΔH), entropy (ΔS), and stoichiometry (n).

2023年6月25日 · Isothermal titration calorimetry (ITC) is a technique for evaluating the thermodynamic profiles of connection between two molecules, allowing the experimental design of nanoparticles systems with drugs and/or biological molecules.

Isothermal Titration Calorimetry (ITC) elucidates the thermodynamic profile (ΔH, ΔS, ΔG, K a, and stoichiometry) of binding and dissociation reactions in solution. While ITC has primarily been used to investigate the thermodynamics of interactions between biological macromolecules and small molecules, it has become increasingly common for ...

Isothermal titration microcalorimetry (ITC) is a common and powerful tool in thermodynamics and related fields, and the connection between the solution behavior and raw titration curve is complex, important, and worth studying, so we try to discuss this problem using a complex solid–solid–liquid diagram.

2025年1月14日 · Isothermal Titration Calorimetry (ITC) is a label-free method for measuring binding of any two molecules that release or absorb heat upon binding. ITC can be used to measure the thermodynamic parameters of biomolecular interactions, including affinity (KA), enthalpy (ΔH), entropy (ΔS), and stoichiometry (n).

Isothermal titration calorimetry (ITC) is a technique used to determine the thermodynamic properties of a chemical or physical equilibria. While the most abundant applications lie in the field of biochemical characterization, its use has increased in other fields because of minimal sample and buffer restrictions.

2016年9月12日 · ITC is a useful tool for studying direct interactions between two molecules in solution — for example, a protein and a small molecule ligand. It can not only inform on whether a binding event occurs, but also provide data on the thermodynamic parameters of the interaction.

In an ITC curve, negative peak refers to "exothermic" interaction, and positive peak means "endothermic". In this sense, how to explain a ITC curve with negative and positive peaks at the same...