The IVTLab is an international and interdisciplinary laboratory promoting cutting-edge research in the field of oncoimmunology and immunotherapy using viruses as platforms to induce and/or orchestrate the tumor-specific immune response.

Learn more about NEB's reagents and kits for in vitro RNA synthesis.

In vitro transcription (IVT) is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities . It is based on the engineering of a template that includes a bacteriophage promoter sequence upstream of the sequence of interest ...

Off-the-shelf kits for in vitro transcription (IVT) of mRNA are commonly used in research and early stages of development. However, for the specific requirements of therapeutics, such kits

In this article, we present an overview of transcription, including requirements of in vitro transcription reactions and a comparison of conventional vs. large scale RNA synthesis. Conventional or large-scale?

Plasmid pT7-hMLH1dn for IVT from Dr. David Liu's lab contains the insert human MLH1dn (codon optimized) and is published in Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. This plasmid is available through Addgene.

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2024年3月25日 · This overview of in vitro transcription (IVT) describes opportunities at each step to steer you toward effective mRNA synthesis. We invite you to download our new E-book, From Template to Transcript, Overcoming Challenges in mRNA Manufacturing, for …

目前，利用体外转录信使RNA(in vitro-transcribed mRNA， IVT mRNA ）进行疫苗接种、基于mRNA的治疗已经成为医学领域的热点，是对抗疾病强大的和通用的工具。mRNA治疗的发展主要包括mRNA的设计、合成、包封、药效学、药代动力学、体内和体外安全性评价、生产和临床 ...

Our IVT platform relies on PCR amplification using a universal primer pair and a plasmid harboring the CDS of interest as template to generate the IVT template. The forward and reverse primers bind to the 5’ and 3’ beta globin UTR, respectively. The forward and reverse primers also encode the T7 polymerase promoter and the polyA tail, respectively.