2021年5月5日 · The results show that 2D 1 H-13 C HSQC NMR is incredibly sensitive to primary, secondary, tertiary, and quaternary structures, and provides unique fingerprints for both the IgG1 and IgG2 subclasses used. Near-ultraviolet circular dichroism (NUV-CD) is also able to differentiate between the two IgG subclasses, while intrinsic fluorescence (FLD ...

2024年9月13日 · The nanobody studied in this work, sdAb-mrh-IgG, was engineered to recognize IgG from rats and mice, but it also weakly recognizes IgG from humans (Pleiner et al. 2018). The development of this antibody followed a traditional protocol, which involved immunizing alpacas and then selecting antigen-specific nanobodies through phage display.

Our mechanistic analysis of this critical quality control step revealed that, unlike all other antibody domains studied, the C H 1 domain of the murine IgG1 heavy chain is an intrinsically disordered protein in isolation. It adopts the typical immunoglobulin fold only upon interaction with its cognate partner, the C L domain.

2024年1月1日 · In our opinion, the 1 H– 13 C HSQC is the best method in the NMR toolbox to resolve glycosidic torsion angles, facilitate the assignment and structure determination process, and confirm topologies hypothesized based on lower resolution data.

2021年5月5日 · In this study, we have compared 2D 1 H-13 C HSQC NMR with two established biophysical methods, i.e., near-ultraviolet circular dichroism (NUV-CD) and intrinsic fluorescence spectroscopy, for the HOS assessments for the folded and unfolded states of two monoclonal antibodies belonging to the subclasses IgG1 and IgG2.

2023年12月20日 · These results agree with HDX-MS findings that the main structural changes upon oxidation in IgG antibodies occur in the C H 2 and C H 3 domains, primarily at the C H 2-C H 3 interface.

The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR.

2007年5月4日 · By virtue of their abilities to produce almost completely nonfucosylated IgG molecules in large quantity, we report the first coordinated X-ray crystallographic and NMR spectroscopic studies of nonfucosylated human IgG1-Fc and compare the results with those of its fucosylated counterpart.

2017年9月27日 · Based on our successful development of isotope labeling of antibody glycoproteins, here we apply NMR spectroscopy to characterize antibody interactions in heterogeneous extracellular environments using mouse IgG-Fc as a test molecule.

An intrinsically quantitative 2D HSQC is self-sufficient for quantifying any molecule having correlation signals on 2D maps. The goal to tackle with intrinsically quantitative 2D HSQC is to reach like with 1D 1H NMR the possibility to derive quantities from the integration of signals by using either an internal or an external