1994年4月8日 · Using a defined RNA polymerase II (pol II) transcription system, we have investigated the roles of basal factors at discrete stages during the transcription cycle (e.g., initiation, promoter clearance, and transcript elongation).

Transcription Factors IIE and IIH and ATP Hydrolysis Direct Promoter Clearance by RNA Poiymeke II James A. Goodrich and Robert Tjian Howard Hughes Medical Institute Department ofMolecular and Cell Biology University of California Berkeley, California 94720 Summary Using a defined RNA polymerase II (pal II) transcrlptlon

2004年4月1日 · In this paper, we show that our recombinant human TFIIE is fully functional to stabilize a pre-initiation complex, consisting of promoter DNA, TBP, TFIIB, RNA polymerase II, and TFIIF. Our recombinant TFIIE also supports TFIIH-dependent and associated ATP-dependent activities in initiation and promoter escape.

1996年4月4日 · We have studied promoter opening in assays reconstituted with purified RNA polymerase II and basal transcription factors. We found that creating a region of heteroduplex DNA around the start site of the adenovirus major late (AdML) promoter circumvents the requirement for TFIIE and TFIIH in transcription.

We propose that IIH functions as a molecular wrench: rotating downstream DNA relative to Þxed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA.

1994年4月8日 · Transcription factor IIE binds preferentially to RNA polymerase Ila and recruits TFIIH: a model for promoter clearance. Genes Dev. 8, 515-524. Recommended articles

1996年4月1日 · We have studied promoter opening in assays reconstituted with purified RNA polymerase II and basal transcription factors. We found that creating a region of heteroduplex DNA around the start site of the adenovirus major late (AdML) promoter circumvents the requirement for TFIIE and TFIIH in transcription.

1999年1月1日 · TFIIH (transcription factor IIH) is a multiprotein complex consisting of nine subunits initially characterized as a basal transcription factor required for initiation of protein-coding RNA synthesis. TFIIH was the first transcription factor shown to harbor several enzymatic activities, likely indicative of functional complexity.

1992年2月5日 · Two new factors required for transcription of class II genes have been identified. These factors, TFIIH and TFIIJ, were required together with the previously described general factors (TFIIA, TFIIB, TFIID, TFIIE, and TFIIF) and RNA …

2000年5月26日 · We propose that IIH functions as a molecular wrench: rotating downstream DNA relative to fixed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA. Human transcription factor IIH consists of …