2022年5月12日 · 首先观察Input组，第一块胶图是利用Anti-Flag检测Flag标签，结果显示第二泳道和第三泳道均检测到条带，说明第二组和第三组实验都存在A-3×Flag蛋白；第二块胶图是利 …

Co-IP （免疫共沉淀，co-inmunoprecipitation）是经典的利用抗体从样品中捕获靶蛋白及其互作蛋白、复合体的一项技术，能够特异性富集所研究的目的蛋白。 由于过程中采用了非变性条 …

IPED Digital Forensic Tool. It is an open source software that can be used to process and analyze digital evidence, often seized at crime scenes by law enforcement or in a corporate …

2011年7月8日 · As part of a MudPIT-based proteomic analysis of the human Mediator complex, we generated human cell lines expressing Mediator subunits with FLAG epitope tags, …

Flag-tagged wild-type and mutant PKM2 protein were expressed in 293T cells and treated with or without NAM and TSA, then purified by IP. The PKM2 enzyme activity was measured and …

2017年11月9日 · The FLAG-LETM1 proteins were isolated by IP with anti-FLAG and eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial …

2021年12月2日 · Bead-bound FLAG-GFP or IMPβ-FLAG was eluted using the FLAG peptide. To prepare IMPα–CBP80-associated mRNPs, the IPs using either α-CBP80 antibody or rIgG …

2020年8月3日 · IRGM interacts directly with TFEB IRGM tagged with green fluorescent protein (GFP–IRGM) co-immunoprecipitated (co-IPed) with FLAG–TFEB (Fig. 2a).

Thus, at times it is necessary to use mammalian expressed PRMTs, which are immunoprecipitated (IPed) to serve as an enzyme source. Usually, PRMTs are fused to a Myc …

2011年11月8日 · The lysates were IPed using anti-Flag (left) or anti-HA (right). The IPed materials and the whole cell extracts (WCE) were analyzed on Western blot using indicated antibodies. …