2003年10月14日 · An IRE-GFP-Based Reporter System for Measuring Localized Translation To visualize and probe for local protein synthesis in living cells, we developed a system that uses a green fluorescent protein (GFP) gene reporter that uncouples the transport of a particular species of mRNA to dendrites from subsequent protein synthesis.

在构建双顺反子或多顺反子载体的各种策略中，来源于脑心肌炎病毒EMCV的 内部核糖体进入位点 (internal ribosome entry site，IRES) 或来源于口蹄疫病毒FMCV的 自剪切多肽2A (self-cleaving 2A peptide，2A) 被广泛应用，实现了在单个载体上同时表达多个基因的目的。 本篇主要介绍内部核糖体进入位点IRES。

2021年5月26日 · Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis. IRP1 binding to target mRNAs is competitively regulated by ISC occupancy.

2024年5月7日 · Here we report that, in mammalian cells, formation of IRE1α clusters is an ER membrane-bound phase separation event that is coupled to the assembly of stress granules (SGs). In response to...

In a 6-hour pig model, we demonstrated that IRE not only enabled ablation of the liver but also enhanced gene transfer of a locally delivered naked GFP plasmid. As verified in pre-treatment specimens, the GFP protein causing green fluorescence is not normally present in the pig liver.

In Caenorhabditis elegans, as in humans, three proteins sense ER stress and activate the UPR: the ribonuclease inositol-requiring protein-1 (IRE-1), the PERK kinase homolog PEK-1 and activating transcription factor-6 (ATF-6). ire-1 is the only ER-stress sensor gene known in yeast.

2020年5月14日 · Exposure of mouse embryonic fibroblasts (MEF) to the DNA damaging agent etoposide, a topoisomerase II inhibitor, triggers mild IRE1α phosphorylation (Fig. 1a) and formation of IRE1α clusters, as ...

2013年12月3日 · PKA-mediated phosphorylation of Rhes modulates iron trafficking. Ras homolog enriched in striatum (Rhes), is a highly conserved small guanosine-5′-triphosphate (GTP) binding protein belonging to the Ras superfamily. Rhes is involved in the dopamine receptor-mediated signaling and behavior though adenylyl cyclase.

The iron responsive element (IRE) discovered by Hentze and colleagues (Hentze et al., 1987) was adapted in such a way that translation of a GFP-based reporter can be induced in...

Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis. IRP1 binding to target mRNAs is competitively regulated by ISC occupancy.