Coupling the Iron-Responsive Element to GFP--An Inducible
2003年10月14日 · An IRE-GFP-Based Reporter System for Measuring Localized Translation To visualize and probe for local protein synthesis in living cells, we developed a system that uses …
内部核糖体进入位点IRES是什么？有什么作用？ - 知乎
在构建双顺反子或多顺反子载体的各种策略中，来源于脑心肌炎病毒EMCV的 内部核糖体进入位点 (internal ribosome entry site，IRES) 或来源于口蹄疫病毒FMCV的 自剪切多肽2A (self-cleaving …
Iron-sulfur cluster deficiency can be sensed by IRP2 and ... - AAAS
2021年5月26日 · Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 …
Mammalian IRE1α dynamically and functionally coalesces with …
2024年5月7日 · Here we report that, in mammalian cells, formation of IRE1α clusters is an ER membrane-bound phase separation event that is coupled to the assembly of stress granules …
Irreversible Electroporation Is a Surgical Ablation Technique that ...
In a 6-hour pig model, we demonstrated that IRE not only enabled ablation of the liver but also enhanced gene transfer of a locally delivered naked GFP plasmid. As verified in pre-treatment …
The ire-1 ER stress-response pathway is required for normal …
In Caenorhabditis elegans, as in humans, three proteins sense ER stress and activate the UPR: the ribonuclease inositol-requiring protein-1 (IRE-1), the PERK kinase homolog PEK-1 and …
Genotoxic stress triggers the activation of IRE1α-dependent
2020年5月14日 · Exposure of mouse embryonic fibroblasts (MEF) to the DNA damaging agent etoposide, a topoisomerase II inhibitor, triggers mild IRE1α phosphorylation (Fig. 1a) and …
PKA modulates iron trafficking in the striatum via small GTPase, Rhes
2013年12月3日 · PKA-mediated phosphorylation of Rhes modulates iron trafficking. Ras homolog enriched in striatum (Rhes), is a highly conserved small guanosine-5′-triphosphate (GTP) …
Iron-dependent regulation of GFP expression in COS cells
The iron responsive element (IRE) discovered by Hentze and colleagues (Hentze et al., 1987) was adapted in such a way that translation of a GFP-based reporter can be induced in...
Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates …
Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally …