2020年1月13日 · Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to engineer a...

Firstly, iRFP has higher molecular brightness and greater photostability. Secondly, iRFP exhibits greater thermodynamic stability, lower pK a value, and higher binding affinity to BV. Thirdly, iRFP has significantly higher effective brightness in cells and in mice since IFP1.4 apoprotein has lower affinity to BV and is not stable without it.

iRFP713 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2011, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

2013年6月16日 · We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are...

2011年8月1日 · Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of...

iRFPs 在哺乳动物细胞和组织中表现出高亮度，适合长期研究。iRFP670 和 iRFP720 能够在活细胞和小鼠中使用标准方法进行双色成像。四种新的 iRFP 和先前设计的 iRFP713 允许在活体小鼠中进行光谱分离的多色成像。

Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its red homologs in protein labeling. Based on structural analysis of the dimer interface, a monomeric bateriophytochrome is identified from a sequence database, and is engineered into a naturally-monomeric IFP (mIFP).

2013年6月16日 · uorescent proteins (fPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared fPs—irfP670, irfP682, irfP702 and irfP720—from bacterial phytochromes. irfPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. irfP670 and irfP720 enable two-color imaging with stand...

因此对于深层脏器成像观察,可以考虑使用近红外荧光蛋白 ( ear-infrared fluorescent protein,iRFP,因为RFP的最大激发和发射波长分别为690nm和713nm。 Einstein医学院解剖与结构生物学副教授 Vladislav Verkhusha博士为了解决这一问题，他的实验室从细菌光敏色素（用于检测光）提取出荧光蛋白，这一荧光蛋白（称为iRFP）能吸收和发射电磁频场的近红外光，该频段的光对于哺乳动物组织而言几乎是透明的。 研究人员把荧光蛋白定位在肝脏中，这一器官由于血 …

Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially …