2021年12月1日 · Through quartile scoring method, immune simulation and molecular docking, four promising targets including lytic transglycosylase IsaA, HlgA, secretory antigen precursor SsaA, and heme uptake protein IsdB were selected as the shortlisted proteins.

2012年2月27日 · Antigens for which there are promising preclinical data on the use of MAbs against S. aureus include IsaA, IsdB, ClfA, and alpha toxin (AT). Antibodies against IsaA, IsdB, and ClfA bind to the surface of bacteria and can limit disease by either promoting opsonophagocytic killing (OPK) or inhibiting the function of the targeted protein (e.g ...

Antibodies against IsaA, IsdB, and ClfA bind to the surface of bacteria and can limit disease by either promoting opsonophagocytickilling(OPK)orinhibitingthefunctionofthe

2011年12月20日 · In this study, we generated a monoclonal antibody targeting the housekeeping protein IsaA, a suggested soluble lytic transglycosylase of S. aureus, and tested its therapeutic efficacy in two experimental mouse infection models. A murine anti-IsaA antibody of the IgG1 subclass (UK-66P) showed the highest binding affinity in Biacore analysis.

To verify possible effects of some of these extracellular proteins on virulence, we tested isdA, isdB, or isaA mutant strains along with the respective parental strains in the G. mellonella infection model.

2020年9月22日 · Certain proteins such as IsaA, IsdB, IsdA, IsdE, IsdH and Chitinase B were related to the killing of larvae. The IsaA protein, a major antigen of S. aureus, was exclusive to the exoproteome of groups that were highly virulent against G. mellonella, in line with the attenuated activity of an isaA deletion mutant.

aureus (immunodominant staphylococcal antigen A (IsaA) , IsdB and SPA ), 2H7 can enhance the opsonophagocytic killing of S. aureus by both mouse and human neutrophils. We evaluated the protective efficacy of 2H7 in both a murine sepsis model and a murine peritoneal infection model.

In this study, we generated a monoclonal antibody targeting the housekeeping protein IsaA, a suggested soluble lytic transglycosylase of S. aureus, and tested its therapeutic efficacy in two experimental mouse infection models. A murine anti-IsaA antibody of the IgG1 subclass (UK-66P) showed the highest binding affinity in Biacore analysis.

2021年10月31日 · IsaA, IsdB, and hypothetical protein (WP_000737711.1) induce the strongest immune responses through activation of Th1 and stimulation of IFN- γ , IgM, IgG1 and IgG2 secretion by the 7th day ...

2018年8月1日 · IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge