J774A.1 is a cell line isolated in 1968 from the ascites of an adult, female mouse with reticulum cell sarcoma. This cell line can be used in immunology research.

J774A.1 (小鼠单核巨噬细胞) (STR鉴定正确) ￥1600. 现单独购买该产品也可享受限时优惠价！ 细胞培养最优解！ 1.该细胞不建议使用胰酶消化，胰酶会刺激分化；2.超过3天不传代细胞容易 …

High-content image analysis (HCIA) is a multi-parameter approach for cytotoxicity screening. This study provides new insights into HCIA-derived response patterns of murine J774A.1 cells and …

2017年2月8日 · Here we focus on two mouse macrophage models that are widely used to study M. tuberculosis infection: BMDMs (primary macrophages) and J774A.1 (a cell line, hereafter …

J774A.1 macrophages at 80% confluency were plated in T25 flasks (400,000 cells per flask) and cultured at 37 °C and 5% CO 2. The cells were polarized to M1 phenotype by incubating with …

2024年4月15日 · J774A.1 cells synthesize large amounts of lysozyme and exhibit low cytolysis but predominantly antibody-dependent phagocytosis. Interleukin-1β is continuously …

2021年5月20日 · J774A.1 TIB-67™ Description Organism: Mus musculus, mouse Cell Type: monocyte, macrophage Tissue: Ascites Age: adult Gender: Female Morphology: macrophage …

For Murine Macrophages J774A.1, it will take 3-4 days to reach 90% confluency. DMEM media consists of phenol red which acts as a pH indicator. if the media turns into light red or yellow …

The activity of caspase 1, 3, 8 and 9 in mouse macrophage cell line J774A.1 infected with BCG or BCG-MSP1C clones for 48 hours. The untreated cells and LPS-stimulated cells were used as …

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines.It has also been used to determine the …

Total RNA isolated from the J774A.1 mouse macrophage cell line (ATCC ® TIB-67™)

2021年12月1日 · In this work, we assessed the effects of particles of mesoporous bioactive glass MBG-75S and mesoporous nanospheres NanoMBG-75S on RAW 264.7 and J774A.1 …

Results showed that the native food strains of Lpb. plantarum were able to modulate the response of J774A.1 murine macrophages through a predominately NOD signaling pathway that reflects …

2016年4月4日 · In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during infection with the most relevant mycobacterial strains for humans …

Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture. Phagocytosis and cytolysis by a macrophage tumour and …

The EVs released by the J774A.1 mouse macrophage cell line, both spontaneously (S-EV) and after infection with Mycobacterium tuberculosis H37Rv (Mtb-EV), were purified by ultra …

Descriptions J774A. 1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and …

What is the J774A.1 cell line? J774A.1 cells were isolated from the ascitic fluid of an adult female BALB/cN mouse with reticulum cell sarcoma. J774A.1 cells exhibit antibody-dependent …

In this study, we investigated, for the first time, the influence of the primary commitment state of macrophages (uncommitted or M1 commitment states induced by LPS and IFN-γ) at the time …

Mouse macrophages (J774A.1 cell line) were purchased from American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine …