2016年12月7日 · The crystal structure of L6 TIR revealed an interface that is spatially distinct to the AE interface, involving the αD and αE helices and the βE strand. In L6 TIR, this region was shown to be important in mediating L6 TIR self-association, which is a requirement for the cell death signaling function of the L6 TIR (Bernoux et al., 2011).

L6 is a Toll/interleukin-1 receptor (TIR) domain-containing NLR from flax (Linum usitatissimum) conferring immunity to the flax rust fungus. Comparison of L6 to the weaker allele L7 identified two polymorphic regions in the TIR and the nucleotide binding (NB) domains that regulate both effector ligand-dependent and -independent cell death ...

These results demonstrate that cell death signaling induced by L6 TIR, RPS4 TIR, and L6MHV can be monitored in both seedling and adult plants, whereas cell death can only be reached in adult plants grown in soil when induced by an overexpression of RPS4 at 21°C.

2011年3月17日 · In this study, we used the L6 protein as a model to characterize plant TIR domain signaling activity and mode of action. Mutational analysis showed that the TIR domain is dispensable for AvrL567 recognition but is required for induction of defense responses.

比如，亚麻的L基因座编码13个NLR蛋白，其中的L6和L11的TIR和 NBS结构域 完全一致，只有LRR有差异，但是它们却可以识别不同的effector。另外，将L6和L10的TIR-NBS结构域与L2的LRR融合，嵌合蛋白具有了L2的识别特异性【4】。

Structure-based studies identified different TIR-domain dimerization interfaces required for signaling of the plant nucleotide-binding oligomerization domain-like receptors (NLRs) L6 from flax and disease resistance protein RPS4 from Arabidopsis.

However, in the last few years, analysis of plant TIR domain structure and function, has revealed an apparent inconsistency. The flax L6 gene encodes a TNL that confers resistance to flax rust. The structure of the L6 TIR domain revealed an interface involved in TIR–TIR self-association that involves the αD and αE helices .

In this study, the crystallization, X-ray diffraction analysis and preliminary structure determination of the TIR domain from the flax resistance protein L6 (L6TIR) are reported. Plate-like crystals of L6TIR were obtained using PEG 200 as a precipitant and diffracted X-rays to 2.3 Å resolution.

L6是来自亚麻（Linum usitatissimum）的Toll /白介素1受体（TIR）域含NLR，可赋予对亚麻锈菌的免疫力。 L6与弱等位基因L7的比较确定了TIR和核苷酸结合（NB）域中的两个多态性区域，该区域调节效应子配体依赖性和非依赖性细胞死亡信号传导以及核苷酸与受体的结合。

2016年12月8日 · The N-terminal Toll/interleukin-1 receptor/resistance protein (TIR) domain has been shown to be both necessary and sufficient for defense signaling in the model plants flax and Arabidopsis. In examples from these organisms, TIR domain self-association is required for signaling function, albeit through distinct interfaces.