2019年3月13日 · Here we report the cryo-electron microscopy structures of human LAT1–4F2hc alone and in complex with the inhibitor 2-amino-2-norbornanecarboxylic acid at resolutions of 3.3 Å and 3.5 Å,...

2024年12月10日 · l -Type amino acid transporter 1 (LAT1)-specific ligands and polyion complexes are used as brain-specific targets to deliver RNA-based drugs across the blood-brain barrier. We characterized an LAT1-targeting antisense oligonucleotide (ASO)-encapsulated nanoparticle, Phe …

2025年1月22日 · Novel strategies have been developed to circumvent the blood–brain barrier, including CSF delivery, intracranial delivery, ultrasound-based methods, membrane transporters, receptor-mediated transcytosis, and nanotherapeutics. These approaches each have their advantages and disadvantages.

Here, we summarize (i) structural insights that help to explain how LAT1 and 4F2hc assemble to transport amino acids across membranes, (ii) the role of LAT1‐4F2hc in key metabolic signalling ...

Due to the significant up-regulation of LAT1 expression in several human tumors, LAT1 has been an attractive transporter for targeted delivery of amino acid-derived anticancer drugs and prodrugs as well as positron emission tomography (PET) probes for cancer diagnosis.

2020年9月22日 · The spectrum of substrates is similar to that of system LAT1 that transports essential NAAs with a concentration gradient. It seems that Na + -LNAA in the abluminal membrane removes essential NAAs from the brain, and thus, provides a mechanism for controlling LNAA flux and concentration in CSF (Hawkins et al., 2013).

2024年9月26日 · L-type amino acid transporter 1 (LAT1) is integral to the transport of large neutral amino acids across the blood-brain barrier (BBB), playing a crucial role in brain homeostasis and the delivery of therapeutic agents. This review explores the multifaceted role of LAT1 in neurological disorders, inc …

2020年1月9日 · CSF injection of drug is the most widely practiced approach to brain delivery that bypasses the BBB. However, drug injection into the CSF results in limited drug penetration to the brain parenchyma, owing to the rapid export of CSF from the brain to blood.

3 天之前 · 2019年3月14日，西湖大学生物学研究所周强研究员团队在Nature发表了题为Structure of the human LAT1–4F2hc heteromeric amino acid transporter complex的论文（第一单位为清华大学），该研究利用冷冻电镜技术，研究者首次解析了人源LAT1–4F2hc复合体整体分辨率3.3Å的 …

2022年1月1日 · In this review, we aimed to introduce the function of amino acids and their transporters in immune cells, with emphasis on L-type amino acid transporter 1 (LAT1), a transporter of large amino acids. Additionally, we discuss the potential of targeting LAT1 for immune disease treatment and cancer immune therapy.