2009年1月23日 · The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC–MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described.

In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column.

In liquid chromatography (LC), the elution strength is the ability of the mobile phase to sweep away the solutes retained on the stationary phase. This strength depends on the nature of the stationary phase and solutes, as well as on the mobile-phase compo- sition (i.e., nature and concentration of the solvents and additives), pH, and column ...

Elution Gradient elution in liquid chromatography is analogous to temperature programming in gas chromatography. Increased eluent strength is required to elute more strongly retained solutes. RP-HPLC (increase organic, make more non-polar). NP-HPLC (increase solvent to …

1999年9月24日 · This paper provides a general overview of flow-cell based IR detection and briefly discusses early solvent-elimination interfaces for LC–FT-IR. A more comprehensive description is given of interface systems which use spraying to induce rapid eluent evaporation, and which basically represent the state-of-the-art in LC–FT-IR.

2020年2月22日 · Fluorinated eluent additives aid the retention of bases, but reduce it for acids. This research focuses on retention mechanisms in a LC column with C18 stationary phase when novel eluent additives (HFIP, HFTB and TFE as well as NFTB and perfluoropinacol) are used.

2014年7月15日 · Using a spray solvent with alkaline pH, negative ions could be directly generated for acidic analytes (e.g., ibuprofen) in acidic LC eluent by DESI, offering a novel protocol to realize “wrong-way around” ionization for LC/MS analysis.

2009年1月23日 · The review presents an overview of studies concerning the effect of eluent composition on the ionization efficiency of ESI, APCI and APPI in LC-MS. Solvent characteristics are discussed in the light of ionization theories, and selected analytical applications are described.

2019年6月5日 · Buffers exhibit their greatest buffering capacity at +/- 1pH unit around the buffer pKa. The closer the eluent pH is to the buffer pKa, the lower the concentration of buffer which needs to be used in order to provide effective resistance to change in pH.

2010年11月30日 · The development of a contactless postcolumn spotter technology capable of rapidly and accurately depositing LC eluent onto another platform (e.g., 1536-well microtiter plates) is described.