2019年7月12日 · However, while the existing literature shows the formation of LC3-positive structures upon GFP-LC3 overexpression in autophagy-null cells from mice (MEFs) 9, 10, 11, 12, we show that...

The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe here how we determine the occurrence of autophagy in vivo using this mouse model.

2013年11月14日 · A recent study described a semi-quantitative method to measure LC3 fluxes in vivo in mice through the visualization of endogenous LC3 protein turnover in non-central nervous system tissues...

2025年2月4日 · Inhibiting Mst1 kinase-mediated LC3 phosphorylation promoted LC3-mediated LFA1 recruitment to the contact surface through direct interaction with RAPL, uncovering an unprecedented integrin...

027139 C57BL/6-Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/J CAG-RFP-EGFP-LC3 transgenic mice express RFP and EGFP in a pH-dependent manner in phagocytic cells following ischemic injury. They are useful for studying the initiation, progression, and resolution of autophagy in a variety of tissues following ischemic injury.

Although electron microscopy has been the gold standard, recent studies of the molecular mechanism of autophagy have led to the development of several marker proteins for autophagosomes, the most widely used of which is LC3, a mammalian homolog of Atg8. These marker proteins allow identification of autophagic structures by fluorescence microscopy.

2017年1月30日 · Transgenic mice expressing autophagy probes for monitoring autophagy. (1) GFP-LC3 mice express EGFP-LC3 for labeling phagophores and autophagosomes. The number of GFP puncta is counted before and after stimulation. (2) tfLC3 mice express RFP-EGFP-LC3 or mCherry-EGFP-LC3 for measuring autophagy flux.

Transgenic GFP-LC3 mice are a valuable tool to investigate autophagy ; however, comparison of homeostatic and disease responses between GFP-LC3 and wild-type (WT) mice has not been done. We examined the effects of GFP-LC3 expression on autophagy, acinar cell function, and experimental pancreatitis.

2009年1月1日 · Although electron microscopy has been the gold standard, recent studies of the molecular mechanism of autophagy have led to the development of several marker proteins for autophagosomes, the most widely used of which is LC3, a mammalian homolog of Atg8. These marker proteins allow identification of autophagic structures by fluorescence microscopy.

The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe here how we determine the occurrence of autophagy in vivo using this mouse model.