LC3B (E5Q2K) Mouse mAb recognizes endogenous levels of total LC3B protein. This antibody detects both type I and type II forms of LC3B. Cross reactivity was not detected with other family members.

Microtubule-associated protein 1 light chain 3 (MAP1LC3), a human homologue of yeast Atg8, is an essential component of autophagy. LC3 plays a critical role in hybrid degradation pathways in which some but not all components of autophagy are coupled with phagocytosis in a process known as LC3-associ …

12135-1-AP targets LC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Although electron microscopy has been the gold standard, recent studies of the molecular mechanism of autophagy have led to the development of several marker proteins for autophagosomes, the most widely used of which is LC3, a mammalian homolog of Atg8. These marker proteins allow identification of autophagic structures by fluorescence microscopy.

2020年6月15日 · We generated a new transgenic mouse model that expresses a pHluorin-mKate2 fluorescent protein fused with human LC3B (PK-LC3 mice) for monitoring autophagy activity in neurons of the central...

027139 C57BL/6-Tg (CAG-RFP/EGFP/Map1lc3b)1Hill/J <i>CAG-RFP-EGFP-LC3</i> transgenic mice express <i>RFP</i> and <i>EGFP</i> in a pH-dependent manner in phagocytic cells following ischemic injury. They are useful for studying the initiation, progression, and resolution of autophagy in a variety of tissues following ischemic injury.

2025年1月13日 · Lc3 over-expression improves survival and attenuates lung injury through increasing autophagosomal clearance in septic mice. LC3B plays a regulatory role in hyperoxia-induced epithelial apoptosis

To establish the structural basis for the recognition of FYCO1 by LC3, the crystal structure of mouse LC3B in complex with the FYCO1 LC3-interacting region (LIR) motif peptide was determined. Structural analysis showed that the flanking sequences N-terminal and C-terminal to the LIR core sequence of FYCO1, as well as the tetrapeptide core ...

We characterized the dynamics of autophagy in vitro using four different cell systems and analyzing markers widely used in this field, i.e. LC3 (microtubule-associated protein 1 light chain 3; protein recruited from the cytosol (LC3-I) to the autophagosomal membrane where it is lipidated (LC3-II)) and p62/SQSTM1 (adaptor protein that serves as ...