Molecular basis of global promoter sensing and nucleosome …
2024年10月1日 · This study elucidates the structural and molecular mechanisms by which the SWR1 chromatin remodeler is specifically recruited to accessible promoter DNA of active or poised genes and subsequently captures the +1 nucleosome for …
Article Molecular basis of global promoter sensing and …
2024年11月27日 · In our model, the Swc3 subunit, along with its basic long helix (LH), imparts a bias for SWR1 to bind the extended stretch of naked DNA present at the NFR of gene promoters. The SWR1 complex exhibits substantially lower affinity for NCPs compared with naked DNA or
Molecular Architecture of the ATP-Dependent Chromatin …
The ATP-dependent chromatin-remodeling complex SWR1 exchanges a variant histone H2A.Z/H2B dimer for a canonical H2A/H2B dimer at nucleosomes flanking histone-depleted regions, such as promoters. This localization of H2A.Z is conserved throughout ...
SWR1 染色质重塑剂全局启动子感应和核小体捕获的分子基础,Cell
2024年10月1日 · SWR1-核小体复合物的完整结构模型说明了 Swc2 和 Swc3 亚基在 SWR1 定向核小体参与中的关键作用。 此外，Swc3 亚基内延伸的 DNA 结合α螺旋能够感应核小体接头长度，并且对于 SWR1 启动子特异性募集和活性至关重要。
Nucleosome flipping drives kinetic proofreading and processivity by SWR1
2024年11月6日 · Here we used single-molecule analysis to demonstrate two-step double exchange of the two H2A–H2B dimers in a canonical yeast nucleosome with Htz1–H2B dimers, and showed that double exchange can be...
SWR1 Chromatin Remodeling Complex: A Key Transcriptional …
2019年12月12日 · SWI2/SNF2-Related 1 Chromatin Remodeling Complex (SWR1-C) belongs to the INO80 chromatin remodeling family and mainly catalyzes the exchange of H2A-H2B with the H2A.Z-H2B dimer. The replacement of H2A.Z into nucleosomes affects nucleosome stability and chromatin structure.
酵母SWR1-核小体复合物的结构和动力学,Science - X-MOL
2018年10月11日 · 荧光探针位于 h2a 组蛋白和 dna 末端，以监测 swr1 和 atp（或 atp 类似物）结合后核小体动力学的变化。结果 我们以 3.6-Å 分辨率确定了 swr1-核小体复合物的冷冻电镜结构。复合体的架构显示了 swr1 复合体是如何围绕 ruvbl1 和 ruvbl2 亚基的异六聚体核心组装的。
Molecular architecture of the ATP-dependent chromatin …
2013年9月12日 · The ATP-dependent chromatin-remodeling complex SWR1 exchanges a variant histone H2A.Z/H2B dimer for a canonical H2A/H2B dimer at nucleosomes flanking histone-depleted regions, such as promoters. This localization of H2A.Z is conserved throughout eukaryotes. SWR1 is a 1 megadalton complex containing …
Nucleosome flipping drives selectivity and processivity of histone ...
2024年2月8日 · By flipping the nucleosome, SWR1 can orientate either of the H2A-H2B histone dimers towards the ATPase motor, primed for exchange. By employing different on-pathway intermediates as substrates we further show that SWR1 has a kinetic preference for presenting a H2A-H2B dimer for exchange over H2A.Z-H2B.
核小体翻转驱动SWR1的动态校对和处理能力 - 科学网
2024年11月6日 · 据介绍，酵母SWR1复合物催化核小体中组蛋白H2A-H2B二聚体与Htz1-H2B二分体的交换。 研究人员使用单分子分析来证明典型酵母核小体中两个H2A-H2B二聚体与Htz1-H2B二分体的两步双交换，并表明双交换可以在不从SWR1复合物中释放核小体的情况下进行。 进一步的分析表明，结合的核小体在两种状态之间翻转，每种状态都呈现出不同的面貌，因此组蛋白二聚体也呈现出SWR1的面貌。 当H2A-H2B二聚体被交换时，结合停留时间比Htz1-H2B二聚合体被 …