液-液相分离（Liquid-Liquid Phase Separation, LLPS）是指在细胞内某些生物大分子（如蛋白质和RNA）通过相互作用，形成具有不同组分和性质的相分离液滴[1]。 这些液滴类似于油滴在水中的分离状态，形成了细胞内的…

LLPS的发生，高度依赖溶液中生物大分子（比如：蛋白，DNA以及RNA等）的浓度、物理化学性质，以及溶液所处的环境，比如：温度、pH、盐离子浓度、盐离子类型以及溶液中存在其它的生物大分子。 作者使用如下的Phase Diagram来描述这些与相分离相关的条件与该溶液是否发生相分离的关系（图2）。 简单来讲：当溶液中所处的大分子浓度低于一个特定的值c时，这一体系无论在什么样的温度，pH等条件下都不能发生相分离。 当高于这一浓度后，在合适的pH以及温度等条 …

2021年8月2日 · Emerging evidence suggests that liquid–liquid phase separation (LLPS) represents a vital and ubiquitous phenomenon underlying the formation of membraneless organelles in eukaryotic cells...

TL;DR: This work presents a detailed characterization of the effects of K+, Mg2+ and nucleosome fiber length on the physical state and property of reconstituteducleosome arrays and demonstrates that the presence of reduced BSA and acetate buffer is not essential for the chromatin LLPS process.

2023年12月1日 · Liquid-liquid phase separation (LLPS) is a captivating phenomenon in which a uniform mixture spontaneously divides into two liquid phases with differing component concentrations.

2020年9月18日 · 生物大分子（蛋白质和RNA）的液-液相分离（Liquid-liquid phase separation， LLPS）是近几年来国际生命科学领域的一个新兴热点。异常的相分离和过渡与多种人类疾病如神经退行性疾病和癌症有因果关系。

生活中可以见到水上漂浮的油滴，就是一种相分离现象。一共两种相，即水和油，由于都是液体，也叫 液液相分离 （LLPS，liquid-liquid phase separation）。 回顾下我们幼儿园学过的知识：

Liquid-liquid phase separation (LLPS) has emerged as a mechanism that has been used to explain the formation of known organelles (e.g. nucleoli, promyelocytic leukemia nuclear bodies (PML NBs), etc) as well as other membraneless condensates (e.g. nucleosome arrays, DNA damage foci, X-chromosome inactivation (XCI) center, paraspeckles, stress gra...

Phase separation, or liquid-liquid phase separation (LLPS), is a ubiquitous and important mechanism for the formation of membraneless condensates in cells. LLPS is driven by multivalent macromolecular interactions, whereas resulting condensates have been regarded as an emerging paradigm of cellular organization ( Banani et al., 2017 ; Boeynaems ...

2021年8月15日 · Liquid-liquid phase separation (LLPS) has emerged as a mechanism that has been used to explain the formation of known organelles (e.g. nucleoli, promyelocytic leukemia nuclear bodies (PML NBs), etc) as well as other membraneless condensates (e.g. nucleosome arrays, DNA damage foci, X-chromosome inac …