2023年9月14日 · 2型糖尿病（Type 2 Diabetes Mellitus,T2DM），是与代谢紊乱相关的慢性疾病，具有持续高血糖和脂质代谢异常的特点，已然成为一种严重的全球健康负担。 T2DM还可以诱发全身多个器官损伤进而导致严重的并发症，例如糖尿病性肾病、视网膜病变、心脑血管疾病和慢性肝损伤等。 糖尿病肝损伤（diabetic liver injury,DLI）是一种慢性肝损伤，也是慢性炎症和糖脂代谢紊乱的一种形式，即使没有其他已知条件，持续高水平血糖和血脂也足以驱动DLI的发生。 长 …

2022年10月1日 · Consistently, YAP1 deficiency aggravated LO2 cells ferroptosis, but YAP1 overexpression alleviated LPS-induced LO2 ferritinophagy, as evidenced by reduced mitochondrial ROS and Fe 2+, along with down-regulated expression of SFXN1 and NCOA4.

PDT已用于临床癌症治疗，它是由光敏剂在特定波长光照射下引发光化学反应产生活性氧物质（Reactive oxygen species, 简称ROS）杀伤肿瘤的治疗方法。 徐抒平教授课题组之前采用SERS技术对PDT治疗后的黑色素瘤细胞核组分（核酸、蛋白质）进行解析（ Front.

2022年2月3日 · Bdh1 knockdown led to ROS overproduction and ROS-induced inflammation and apoptosis in LO2 cells, while Bdh1 overexpression protected LO2 cells from lipotoxicity by inhibiting ROS...

2023年8月1日 · C3G–LA up-regulated the expression levels of Nrf2 and the downstream expression levels of NQO1 and HO-1 in H 2 O 2-stressed LO2 cells, inhibited the excessive production of intracellular ROS and MDA, restored MMP, enhanced antioxidant enzyme activity, improved cell membrane integrity, inhibited cellular apoptosis and finally ameliorated cell ...

HepG2细胞和LO2细胞均使用含10%胎牛血清的DMEM培养基，置于37℃、5%的CO2培养箱中培养，待细胞汇合度达到70%~80%时，用0.25%胰酶-EDTA消化细胞，按1：3传代。 取对数生长期的HepG2细胞和LO2细胞进行实验。 3.2建模. HepG2细胞和LO2细胞铺板18h后，将培养基换为含5%胎牛血清、0.5%无脂肪酸牛血清白蛋白的DMEM培养基。 将两种细胞系暴露于PA、OA或其1：2混合物诱导处理24h、48h，进行CCK8测定细胞存活率、甘油三酯酶法检测，实验重复3 …

We compared the reactive oxygen species (ROS) levels between CHO-treated cells and control groups in LO2 cells by flow cytometry. The results showed that CHO-treated LO2 cells upregulated the ROS levels compared to the normal control (NC) group (Figure 4A and 4B).

These results indicated that crocetin prevented LO2 cells from TGF-β-induced injury by regulating ROS, SOD, MDA, and GSH levels, as well as COL1A1, TGF-β, α-SMA, and NDRG2 levels. Crocetin Promotes Cell Autophagy via AMPK/m-TOR Signaling Pathway

2023年6月1日 · ROS not only stimulate caspase-9 and further activate caspase-3, which leads to the fragmentation of PARP, but also decrease the expression of the JAK-2 protein and STAT-3 protein. Finally, ROS induce the endogenous apoptosis pathway regulated by mitochondria and inhibit the growth and survival of tumor cells.

吉林大学徐抒平教授通过荧光成像技术监测了经过不同剂量的光动力治疗 (PDT)后的线粒体微环境，包括线粒体膜电位 (MMP)和活性氧 (ROS)，并利用三种细胞系 (HepG2、MCF-7和LO2)进行了比较。 同时，实验也利用线粒体靶向的表面增强拉曼散射 (SERS) pH纳米传感器对线粒体内的pH波动情况进行了研究。 本文要点： （1）实验结果表明，随着活性氧的产生，MMP也会逐渐降低，而癌细胞对过量ROS的反应相对于正常细胞来说也更弱。 （2）与此同时，线粒体内的pH值也 …