2024年3月1日 · RNA-Seq analysis of FRC-Exo-treated primary kidney tubular cells (PKTCs) revealed that FRC-Exos influenced PKTC fate in the presence of lipopolysaccharide (LPS). FRC-Exos promoted kinase PINK1-dependent mitophagy and inhibited NLRP3 inflammasome activation in LPS-stimulated PKTCs.

FRC-Exos promoted kinase PINK1-dependent mitophagy and inhibited NLRP3 inflammasome activation in LPS-stimulated PKTCs. To dissect the mechanism underlying the protective role of Exos in S-AKI, we examined the proteins within Exos by mass spectrometry and found that CD5L was the most upregulated protein in FRC-Exos compared to macrophage ...

2023年12月30日 · In this study, we demonstrated that FRC-Exos improved kidney function in S-AKI. FRC-Exos, which are rich in CD5L, promoted PINK-Parkin–mediated mitophagy in primary kidney epithelial cells, resulting in the degradation of the NOD-like receptor protein 3 (NLRP3) inflammasome and improved kidney function. Furthermore, modification of

2014年10月27日 · Fibroblastic reticular cells (FRCs) are LN-resident mesenchymal cells that secrete and remodel the extracellular matrix to create a dense reticular network....

FRC-Exos 在 LPS 刺激的 PKTC 中促进激酶 PINK1 依赖性线粒体自噬并抑制 NLRP3 炎性体激活。为了剖析 Exos 在 S-AKI 中的保护作用背后的机制，我们通过质谱法检查了 Exos 内的蛋白质，发现与巨噬细胞衍生的 Exos 相比，CD5L 是 FRC-Exos 中上调最多的蛋白质。

2019年8月5日 · Fibroblastic reticular cells (FRCs), a subpopulation of stromal cells in lymphoid organs and fat-associated lymphoid clusters (FALCs) in adipose tissue, play immune-regulatory roles in the host response to infection and may be useful as a form of cell therapy in sepsis.

2024年2月22日 · 淋巴结中富含成纤维网状细胞（FRC），其与免疫细胞存在着广泛的正向相互作用，可促进淋巴管的生成和T细胞的存活。 最近有研究发现 FRC 移植在小鼠脓毒症中显示出良好的治疗效果，然而FRC 减缓 S-AKI 的确切机制仍不清楚。 此外，双膜结合囊泡的外泌体在介导细胞间通讯中发挥着重要作用，探究FRC衍生的Exos（FRC-Exos）在S-AKI中的功能，可为AKI（S-AKI）的治疗与预防研究提供新见解。

目的 探讨纤维网状细胞 (FRC)通过促进线粒体凋亡对脓毒症急性肾损伤 (AKI)的改善作用.方法 取10～12周龄的C57BL/6雄性小鼠分别进行小鼠原代肾小管上皮细胞 (TECs)及小鼠肠系膜脂肪组织来源的FRC的分离培养,采用流式细胞术鉴定FRC和原代TECs、免疫荧光检测TECs AQP1的表达、油红染色鉴定FRC的干细胞特性.将TECs分为NC组、LPS组与LPS-FRC组,采用流式细胞术检测3组TECs JC-1单体百分比、qRT-PCR检测炎症因子 [白细胞介素 (IL)-1和IL-6]mRNA表达水平 …

We describe a population of immune-specialized myofibroblasts derived from lymph node tissue, termed fibroblastic reticular cells (FRCs). Because FRCs have an immunoregulatory function in lymph nodes, we hypothesized that ex vivo–expanded FRCs would control inflammation when administered therapeutically.

2023年5月1日 · We have shown that Fibroblastic Reticular Cells (FRC)-based therapy improves outcome in a mouse polymicrobial sepsis model. Modulating TLR4 and TLR9 signaling in FRC regulates the efficacy of FRC-based therapy in sepsis.