脂多糖（Lipopolysaccharide, LPS）是革兰氏阴性细菌外膜的主要成分之一，由类脂A、核心多糖和O-抗原三部分组成，其中O-抗原决定了LPS的血清型，具有重要的生物学功能和作用机制。

SFA结果表明，在室温（RT）下，LPS-Ra囊泡在纯水中微弱地吸附到云母上，并且当两个表面彼此接近时，表面层很容易被挤出。 但是，在室温下以接近生理浓度（2.5 mM）添加钙（Ca 2+）阳离子时，会观察到LPS多层或云母上的LPS脂质体变形，这可能是由于LPS磷酸基团之间以及LPS磷酸酯和带负电的云母之间的桥接由Ca 2+介导，在表面分离D 0处有硬壁排斥约30–40 nm。 在40℃，这是上面的LPS镭β-α酰基链熔融温度（Ť米= 36℃），可以观察到在压缩下的表面层 …

2022年11月1日 · Lipopolysaccharide (LPS) is a cell-wall component of Gram-negative bacteria which contributes to bacterial toxicity. During processes such as cell division, shedding of outer membrane vesicles, or bacterial cell death, LPS is released into the surrounding media.

Ra、Rb、Rc、Rd、Re等（其中a、b、c等分别表示第一、第二、第三度等）表示给定LPS的多糖长度。 Ra和Re分别表示链最长和最短的突变体。 2最极端的突变体是Re突变体，它产生的LPS由脂质A和3-脱氧-D-甘露-辛酮糖酸（2-酮基-3-脱氧辛酸， KDO ）组成，是核心的唯一组成 ...

2019年12月3日 · Complementary dynamic light scattering (DLS) characterization suggests that vesicle-like aggregates of diameter ∼28-80 nm are formed by LPS-Ra in aqueous media. SFA results show that LPS-Ra vesicles adsorb weakly onto mica in pure water at room temperature (RT) and the surface layers are readily squeezed out as the two surfaces approach each ...

2023年10月1日 · Combining DLS, AFM and surface force apparatus (SFA), the authors demonstrated that LPS-Ra formed vesicle-like aggregates formed by in aqueous media, which adsorbed on the negatively charged mica surface mediated by Ca 2+. LPS membrane fusion was reported for the first time using SFA above the LPS-Ra acyl chain melting temperature.

2025年2月1日 · The smooth LPS from S. minnesota consisting of the highest polysaccharide content, is masked most rapidly, followed by the Ra and Rb mutant with the second and third highest polysaccharide content. Although the Ra and Rb mutants exhibited recoveries above 50 % after 45 min in two out of three assays, they showed a tendency to lower recoveries ...

The structural polymorphisms of rough mutant lipopolysaccharides (LPS) Rd, Rc, Rb, and Ra from Salmonella minnesota (strains R4, R7, Rz, R5, R345, and R60, respectively) were investigated as a function of temperature, water content, and Mg2+ concentration.

2013年9月10日 · We have previously reported that bacterial toxins, especially endotoxins such as lipopolysaccharides (LPS), might be important causative agents in the pathogenesis of rheumatoid arthritis (RA) in an in vitro model that simulates the …

2015年7月2日 · We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence of calci