2024年12月11日 · Placenta-derived sFlt-1 is upregulated during pre-eclampsia and sequesters vascular endothelial growth factor (VEGF), hindering vasodilation. No therapeutic has been clinically...

In vitro activation of macrophages by LPS induces rapid release of vascular endothelial growth factor (VEGF) and soluble fms-like tyrosine kinase-1 receptor (sFlt-1), which are thought to be the effectors to cause sepsis. However, the signal pathway that controls the VEGF and sFlt-1 expressions in LPS-activated macrophages remains unclear.

本研究通过脂多糖（LPS）及亚硝酸左旋精氨酸甲酯（L-NAME）诱导建立不同的PE样小鼠模型，对不同的PE样小鼠模型母体血清、肝脏及胎盘组织中Pra对sFlt-1、PlGF、VEGF的不同作用的并行研究，从血管生成平衡的影响方面进一步阐释PE发病的多因素-多机制-多通路问题。

2024年1月30日 · The results showed that silencing FosB significantly reversed the decrease in the cell viability, the cell migration speed, and the concentration of IL-6, TNF-α, and sFlt-1 caused by LPS stimulation (Fig. 2 C–E). FosB silencing significantly reduced the expression of LPS-stimulated p–NF–κB/NF-κB and Flt-1 levels (Fig. 2 F–H). These ...

Soluble fms-like tyrosine kinase (sFlt-1) is an important mediator in the pathogenesis of preeclampsia. We sought to determine if platelet-monocyte aggregates (PMAs) produced sFlt-1 and if PMAs contributed to sFlt-1 production in preeclampsia.

2018年11月30日 · Eighty Male BALB/c mice were randomly assigned to four group: PBS group, LPS group, sFlt group, or LPS + sFlt group. The levels of vascular endothelial growth factor and total protein in bronchoalveolar lavage fluid were compared, together with lung injury score and the histopathology of alveolar epithelial barrier.

Lipopolysaccharides (LPS) are present on the outer membrane of Gram-negative bacteria and binds toll-like receptor 4 complex in numerous cell types. In immune cells, LPS can trigger the release of proinflammatory cytokines and the generation of reactive oxygen species (Mittal et al., 2014). LPS are the prototypical endotoxin and can induce ...

本論文研究結果發現廣效性的PKC抑制劑GF109203X可以抑制LPS誘導老鼠吞噬細胞RAW264.7或是人類周邊血液的吞噬細胞分泌sFlt-1，但促進了LPS所誘導VEGF的分泌。 此外將細胞以專一性PKCδ抑制劑Rottlerin處理，或在細胞內表現無酵素活性的PKCδ (K376R)突變蛋白也可以得到相同的結果，顯示PKCδ在LPS誘導sFlt-1和VEGF分泌的分子機制中，分別扮演了正面與負面調控的角色。 Statin是 HMG-CoA reductase的抑制劑，臨床上主要用來抑制中間產物mevalonate的生 …

2024年1月17日 · We found that huc-Exos-MSC-derived miR-144 alleviated gestational hypertension and inflammation in PE-like pregnant rats by regulating the FosB/Flt-1 pathway. In addition, huc-Exos-MSC-derived miR-144 could partially reverse the LPS-induced adverse pregnancy outcome and brain injury in fetal rats, l …

腹腔注射 R848 造模法较尾静脉注射 LPS 法对动物的损伤程度更严重,且给药方式为腹腔注射,操作简单。 Objective To compare and analyze differences between two modeling method of mouse pre-eclampsia (PE), and to provide a reference to select different types of PE animal models.