Overexpression of CD14, TLR4, and MD-2 in HEK 293T cells does …
Human HEK 293T cells are completely unresponsive to LPS, but acquire high LPS sensitivity following transient transfection with CD14, TLR4, and MD-2 (293T/CD14/TLR4/MD-2 cells), as …
Toll-like Receptor-4 Mediates Lipopolysaccharide-induced Signal ...
1999年4月16日 · LPS increases TLR4-mediated NF-κB activation in a dose- and time-dependent manner. HEK 293 cells were transiently transfected with TLR4 cDNA or vector DNA and …
Intracellular galectin-3 is a lipopolysaccharide sensor that ... - Nature
2022年12月8日 · Strikingly, treatment of glucose/amino acids-deprived HEK-293T cells with E. coli LPS (O111:B4), a complex of lipids and polysaccharides containing β-galactoside …
Can LPS induce inflammation in HEK293 cells? - ResearchGate
2022年7月1日 · I've been trying to induce an inflammatory response using LPS (Sigma O127:B8 and O55:B5) using the immortalized human myometrial cells (TERT-HM) in our lab but have …
Deficient chaperone-mediated autophagy facilitates LPS-induced ...
2023年10月6日 · Upon stimulation with lipopolysaccharide (LPS), the transcription factor NF-κB/p65 enters the nucleus to transactivate genes that express proinflammatory cytokines and …
USP18 negatively regulates NF-κB signaling by targeting TAK1
2015年8月4日 · In this study, we identified a member of the deubiquitinases family, ubiquitin-specific protease 18 (USP18), as a novel negative regulator in Toll-like receptor (TLR) …
We found that overexpression of CD14, TLR4, and MD-2 confers LPS sensitivity to other-wise LPS-unresponsive HEK 293T cells, but does not pre-vent induction of LPS tolerance, despite …
LPS-induced activation of NF-kB in human embryonic kidney cells (HEK293) (5, 6), whereas in 293T and Ba/F3 cells, TLR4-mediated responses to LPS requires the presence of MD-2, a cell …
Impact of lipopolysaccharides on cultivation and recombinant …
2021年10月25日 · In this study LPS from E. coli (Figure 1) was added to HEK-293 cell cultures to evaluate the impact on cell viability and recombinant protein production. The LPS used was a …
An examination of the LPS-TLR4 immune response through the …
3 天之前 · The LPS-TLR4 immune response is a critical mechanism in the body's defense against Gram-negative bacterial infections, yet its dysregulation can lead to severe inflammatory …