A plaque assay can be used to enumerate viruses that lyse their host cells. In a plaque assay the host cells and virus are incubated together for a short time to allow the virus to attach to and enter the host cell.

Plaque assays remain one of the most accurate methods for the direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture. Here we ...

2025年3月21日 · In this lab, we will be quantifying bacteriophages (viruses that attack bacteria) using a plaque assay. This means that we must grow both bacteria and virus. As the virus replicates and grows, it will lyse bacteria resulting in an …

The purpose of the plaque assay is to grow isolated plaques of phage particles within a lawn of bacteria. Students can visualize the clearing of bacterial growth on the agar media, demonstrating the effect of phage on bacteria. This method also requires that students perform serial dilutions,

As the virus replicates and grows, it will lyse bacteria resulting in an area of clearing that can be observed on a bacterial lawn. These clearings are called plaques. A plaque assay, therefore, is a method by which the number of plaques observed can be used to estimate the amount of virus present in the original culture.

A plaque is an area of clearing in a confluent lawn of bacterial growth which represents the spot where a virus has landed, infected the bacteria it encountered, and lysed them. Each plaque represents a virus particle or plaque-forming unit (PFU).

The plaque assay is a well-established method for measuring virus concentration as it relates to infectious dose. The assay relies on determining the number of plaque-forming units (PFU) created in a monolayer of virus-infected cells.

2023年8月24日 · It's an effective method used to measure viral concentration, particularly applicable to bacteriophages (viruses that infect bacteria). It's based on the principle that any virus, when plugs into a cell, will result in the formation of clear zones called "plaques" on …

Plaque assay is one of the widely used approaches for determining the quantity of infectious virus in a sample. Only viruses that cause visible damage to cells can be assayed in this way. Plaque assay was first developed to calculate the titers of bacteriophage stocks.

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