2024年2月5日 · LAMP1 was fused to a SBP and a fluorescent protein in its luminal domain and transiently expressed in HeLa cells. In the absence of biotin, SBP-mCherry-LAMP1 was retained in the ER, thanks to the coexpression of the ER-resident Streptavidin-KDEL …

2023年4月6日 · HeLa cells expressing doxycycline-inducible LAMP1–APEX2 were treated with LLOMe followed by exposure to biotin and H 2 O 2.

2021年12月17日 · The protocol below describes the experimental steps required for using HeLa cells stably expressing the lysosomal protein marker Lamp1 tagged with a monomeric form of the green fluorescent protein (mGFP), which allows one …

2018年1月24日 · Confocal microscopy imaging of a stable mitochondria-lysosome contact which remains in contact for over 1 min (60 sec) in a living Hela cell expressing Lamp1-mGFP (lysosome; green) and mApple...

Invitrogen Anti-LAMP1 Monoclonal (H4A3), Catalog # MA5-18121. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate samples.

研究人员首先通过荧光探针监测u2os细胞，发现其溶酶体蛋白水解活性在饥饿反应中显著增加。lamp1标记的溶酶体在hela和u2os细胞中在核周围聚集，并且大部分不运动。在饥饿细胞的核周区域电子密集度大的可能是降解的溶酶体。

2023年7月11日 · We found that LAMP1 or LAMP2 KD dramatically reduced hypoxia-induced MMEL but had no effect on megamitochondria formation in HeLa cells (Figs. 3e, 3f, and S4f–j), indicating that lysosomal ...

2020年1月6日 · Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines.

HeLa cells transiently expressing GFP-or YFP-tagged candidates were fixed, immunostained with an antibody directed against LAMP1, a late endosome and lysosome marker, and imaged by...

2004年7月13日 · We now report the trafficking of a synthetic protein designed to facilitate analysis of the post-Golgi movement of Lamp1. To restrict labeling to the late Golgi, the lumenal domain of the chimera contains two tyrosine sulfation motifs.