2021年6月17日 · Using ex vivo and in vivo experiments, we demonstrate that FYN-TRAF3IP2 and KHDRBS1-LCK activate signaling pathways downstream of the T cell receptor (TCR) complex and confer therapeutic...

CD28 CD was expressed as a glutathione S-transferase (GST) fusion protein and purified after cleavage of the GST fusion partner. Purified recombinant Lck readily phosphorylated CD28WT CD in vitro in the presence of adenosine 5′-triphosphate (ATP) and metal cofactors, as shown by Western blotting analysis with an antiphosphotyrosine antibody ...

2020年7月20日 · We report a previously unknown binding motif in the CD3ε cytoplasmic tail that interacts in a noncanonical mode with the Lck SH3 domain: the receptor kinase (RK) motif. The RK motif is accessible...

2024年11月7日 · Gao and Skolnick use deep learning to identify protein-protein interaction partners of tyrosine kinase Lck among ∼1,000 immune-related proteins. Predicted structures for several complexes reveal potential molecular mechanisms, providing insights into their functions in T cell regulation and signaling.

2017年6月28日 · CD3ε−Lck interaction was crucial for total TCR phosphorylation. HEK293FT cells with 1G4 TCR and Lck WT or Lck D12N mutant expression were stimulated by α-CD3 antibody (OKT3, eBioscience) for the indicated time, and then cells were lysed for Western blot analysis. Lck had a C-terminal mCherry tag, so it migrated at 70 kDa.

2021年1月21日 · Applying network-based systems pharmacology analyses to examine signal circuitry, we identified preTCR–LCK activation as the driver of dasatinib sensitivity and T-ALL-specific LCK dependency...

2021年6月17日 · Using ex vivo and in vivo experiments, we demonstrate that FYN-TRAF3IP2 and KHDRBS1-LCK activate signaling pathways downstream of the T cell receptor (TCR) complex and confer therapeutic vulnerability to clinically available drugs.

We have expressed the protein-tyrosine kinase p56lck as a fusion with glutathione-S-transferase (GST) or thioredoxin (TRX). While the GST-Lck fusion protein was found to be poorly soluble and solubilization techniques led to a high degree of degradation, the TRX-Lck fusion protein was found to be hi …

2020年6月22日 · Src family kinase Lck plays critical roles during T cell development and activation, as it phosphorylates the TCR/CD3 complex to initiate TCR signaling. Lck is present either in coreceptor-bound or coreceptor-unbound (free) forms, and we here present evidence that the two pools of Lck have different molecular properties.

This modified GFP is myristoylated and targeted to the plasma membrane (rafts). It was constructed from a parent Lck-GFPN1 construct. The parent Lck sequence was removed and replaced with a new Lck sequence containing two glycine residues at the C-term of Lck, as opposed to an isoleucine and a valine.