Disrupting the LC3 Interaction Region (LIR) Binding of Selective ...
2020年3月31日 · Our results demonstrate that inhibition of LIR of a single autophagy receptor is insufficient to sensitize the cells to cytarabine, while simultaneous inhibition of three LIR motifs in three distinct SARs reduces the IC 50 of the chemotherapeutic.
Critical assessment of LC3/GABARAP ligands used for degrader
2024年11月25日 · LC3/GABARAP recruit cargo-receptor-complexes by a short sequence motif within the receptors called the LIR (LC3-Interacting Region), mediating autophagosomal recruitment and degradation by ...
The LIR motif – crucial for selective autophagy
2013年8月1日 · A main driving force has been the discovery of specific autophagy receptors that sequester cargo into forming autophagosomes (phagophores). At the heart of this selectivity lies the LC3-interacting region (LIR) motif, which ensures the targeting of autophagy receptors to LC3 (or other ATG8 family proteins) anchored in the phagophore membrane.
Selective Autophagy: ATG8 Family Proteins, LIR Motifs and Cargo ...
2020年1月3日 · The cargo-bound receptors interact with lipidated ATG8 family proteins anchored in the membrane at the concave side of the forming autophagosome. The interaction is mediated by 15- to 20-amino-acid-long sequence motifs called LC3-interacting region (LIR) motifs that bind to the LIR docking site (LDS) of ATG8 proteins.
The LIR motif - crucial for selective autophagy - PubMed
2013年8月1日 · At the heart of this selectivity lies the LC3-interacting region (LIR) motif, which ensures the targeting of autophagy receptors to LC3 (or other ATG8 family proteins) anchored in the phagophore membrane.
Molecular determinants regulating selective binding of autophagy ...
2019年5月3日 · Mammalian ATG8 proteins drive autophagosome formation and selective removal of protein aggregates and organelles by recruiting autophagy receptors and adaptors that contain a LC3-interacting...
Exploring selective autophagy events in multiple biologic ... - Nature
2022年5月10日 · Here, we report the development of molecular traps based in the tandem disposition of LC3-interacting regions (LIR). The estimated affinity of LC3-traps for distinct recombinant LC3/GABARAP...
The crystal structure of mouse LC3B in complex with the FYCO1 LIR ...
To establish the structural basis for the recognition of FYCO1 by LC3, the crystal structure of mouse LC3B in complex with the FYCO1 LC3-interacting region (LIR) motif peptide was determined. Structural analysis showed that the flanking sequences N-terminal and C-terminal to the LIR core sequence of FYCO1, as well as the tetrapeptide core ...
Development of LC3/GABARAP sensors containing a LIR and a …
To develop new sensors for detecting endogenous LC3/GABRARAP‐positive autophagosomes, we constructed sensors containing a short hydrophobic LC3‐interacting motif (LIR). Many LC3‐interacting proteins, such as selective autophagy receptors including p62, contain a basic hydrophobic LIR motif with the core consensus sequence (W/F/Y)XX(L/I/V ...
We demonstrate that each of the human LC3 isoforms (LC3A, LC3B, and LC3C) interacts with Atg13 via the LC3 interacting region (LIR) of Atg13. Using X-ray crystallography, we solved the macromolecular structures of LC3A and LC3C, along with the complex structures of the LC3 isoforms with the Atg13 LIR.