RhoA binds to Rhophilin and regulates the actin cytoskeleton. RhoA also interacts with a Rho target protein, Rhotekin through the RBD motif. RhoA-dependent signaling is recognized as an essential regulator of vascular function and seems to play an important role in major arterial diseases such as hypertension, atherosclerosis and pulmonary ...

2018年3月1日 · We compared the sensitivity and concordance of next-generation sequencing (NGS), droplet digital PCR (ddPCR) and peptide nucleic acid-locked nucleic acid (PNA-LNA) clamp method for detecting the G17V RHOA mutation.

2013年1月1日 · In this review, we focus on the ability of S1P and LPA to signal through regulation of RhoA activity, and the molecular mechanism by which RhoA controls enzymes involved in, and regulated through, lipid metabolism. We consider the role of this pathway in myocardial I/R injury and briefly discuss its potential role in neuroinflammation. 2.

2024年11月20日 · Ras同源基因家族蛋白A (Ras homolog gene family member A, RhoA)/Rho相关卷曲螺旋蛋白激酶(Rho - associated coiled - coil forming protein kinase,ROCK)信号通路是缺血性卒中病理过程中的重要调节因子,其中RhoA是Rho家族的小的鸟苷三磷酸(guanosine triphosphate, GTP)酶,可介导细胞间黏附和细胞 ...

To study the role of RhoA and Rho kinase more specifically, endothelial cells were stimulated with lysophosphatidic acid (LPA), a commonly used RhoA activator. LPA induced a 2- to 3-fold increase in the passage of horseradish peroxidase (HRP) across endothelial monolayers that lasted for several hours, whereas thrombin induced a 5- to 10-fold ...

2018年1月30日 · RhoA GTPase在调节细胞骨架蛋白，细胞形态和迁移以及细胞中各种增殖和转录活性方面发挥多种功能。 RhoA活性受鸟嘌呤核苷酸交换因子（GEF），GTPase活化蛋白（GAP）和鸟嘌呤核苷酸解离因子（GDI）调节。 RhoA-RhoGDI复合物存在于细胞质中，活性GTP结合形式的RhoA位于膜上。 包括IκB激酶γ（IKKγ）在内的GDI置换因子（GDF）使RhoA-GDI复合体解离，从而允许通过GEF激活RhoA。 此外，RhoA中Tyr42磷酸化和Cys16 / 20氧 …

In the present study, we carried out extensive analyses of G17V RHOA mutations using droplet digital PCR (ddPCR), peptide nucleic acid-locked nucleic acid (PNA-LNA) clamp method, and next-generation sequencing (NGS) to clarify the concordance and sensitivity of these methods.

Rho 家族是小GTP结合蛋白家族的成员,调控包括细胞骨架动力学、细胞形态、膜转运和基因表达等各种细胞过程,其主要包括Rho(A,B 和C),Rac(1、2 和3) 和Cdc42[6,7]。 目前,研究最全面的是Rho 家族中的一个小GTPase 蛋白RhoA。 RhoA 蛋白在包括正常人组织、胚胎组织和干细胞[8]在内的所有组织中表达,主要定位在细胞质和质膜以及细胞-细胞接触和细胞突起附近,并在多种细胞过程中发挥重要作用。 Rho相关卷曲螺旋激酶(Rho-associated coiled helix kinase,ROCK)是经典丝 …

2016年12月1日 · MiR-31 inhibited RhoA expression by binding to 3′UTR of mRNA, whereas miR-31 mimics significantly decreased the number of invaded and migrated cells (p<0.05). The activation of RhoA, ROCK, and phosphorylation of MLC remarkably exacerbate the invasion and migration ability of GC cells (p<0.05).

We compared the sensitivity and concordance of next‐generation sequencing (NGS), droplet digital PCR (ddPCR) and peptide nucleic acid‐locked nucleic acid (PNA‐LNA) clamp method for detecting the G17V RHOA mutation.