2018年10月30日 · We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these...

2023年11月1日 · We combined Acr with the light-oxygen-voltage 2 (LOV2) photosensor from Avena sativa according to previous studies (Supplementary Figure S4A) (29, 30). We also optimized the PICI system to simplify the previous procedures by redesigning the complete plasmid for expressing Cas9 and Acr using araBAD and …

2020年3月16日 · In the absence of light, AcrIIA4-LOV2 can bind and inhibit SpyCas9, but upon photoexcitation, the LOV2 domain loses its structural conformation and causes AcrIIA4 to misfold and lose affinity...

We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.

2023年7月1日 · More recently, a separate group showed that circularly permuted LOV2 (cpLOV2, which can provide distinct “caging surfaces” relative to the non-permuted domain) can also control anti-CRISPR functions, 39 affording additional options for effective fusions to the ever-growing roster of Acr proteins.

2020年12月16日 · Here, we report the first optogenetic tool to control NmeCas9 activity in mammalian cells via an engineered, light-dependent anti-CRISPR (Acr) protein. Building on our previous Acr engineering work, we created hybrids between the NmeCas9 inhibitor AcrIIC3 and the LOV2 blue light sensory domain from Avena sativa.

通过 诱导型启动子 、光或小分子调控Acr蛋白，可以实现CRISPR-Cas活性的快速、动态和空间控制。例如，将AcrA4II融合到光敏LOV2结构域，使得抗CRISPR活性可以被蓝光控制(Bubeck等,2018)。 降低CRISPR-Cas编辑在组织中的细胞毒性

2020年4月13日 · Anti-CRISPR (Acr) proteins are powerful tools to control CRISPR–Cas technologies. However, the available Acr repertoire is limited to naturally occurring variants. Here, we applied...

2024年11月25日 · Anti-CRISPR (Acr) proteins, bacteriophage-derived, potent CRISPR-Cas inhibitors, represent a powerful, naturally occurring regulatory layer for CRISPR control. First described in 2013 by Alan perpetuity. It is made available under aCC …

2023年12月11日 · Anti-CRISPR (Acr) proteins are encoded by mobile genetic elements to overcome the CRISPR immunity of prokaryotes, displaying promises as controllable tools for modulating CRISPR-based applications. However, characterizing novel anti-CRISPR proteins and exploiting Acr-related technologies is a rather …