Engineered anti-CRISPR proteins for optogenetic control of
Oct 30, 2018 · We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. …
Rapid characterization of anti-CRISPR proteins and optogenetically ...
Nov 1, 2023 · We combined Acr with the light-oxygen-voltage 2 (LOV2) photosensor from Avena sativa according to previous studies (Supplementary Figure S4A) (29, 30). We also optimized …
Anti-CRISPR protein applications: natural brakes for CRISPR …
Mar 16, 2020 · In the absence of light, AcrIIA4-LOV2 can bind and inhibit SpyCas9, but upon photoexcitation, the LOV2 domain loses its structural conformation and causes AcrIIA4 to …
Engineered anti-CRISPR proteins for optogenetic control of ... - PubMed
We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. …
Applications of Anti-CRISPR Proteins in Genome Editing and ...
Jul 1, 2023 · More recently, a separate group showed that circularly permuted LOV2 (cpLOV2, which can provide distinct “caging surfaces” relative to the non-permuted domain) can also …
Optogenetic control of - Oxford Academic
Dec 16, 2020 · Here, we report the first optogenetic tool to control NmeCas9 activity in mammalian cells via an engineered, light-dependent anti-CRISPR (Acr) protein. Building on …
CRISPR-Cas系统的开关-抗CRISPR蛋白 - 知乎 - 知乎专栏
通过 诱导型启动子 、光或小分子调控Acr蛋白，可以实现CRISPR-Cas活性的快速、动态和空间控制。例如，将AcrA4II融合到光敏LOV2结构域，使得抗CRISPR活性可以被蓝光控制(Bubeck …
Computational design of anti-CRISPR proteins with improved ... - Nature
Apr 13, 2020 · Anti-CRISPR (Acr) proteins are powerful tools to control CRISPR–Cas technologies. However, the available Acr repertoire is limited to naturally occurring variants. …
Nov 25, 2024 · Anti-CRISPR (Acr) proteins, bacteriophage-derived, potent CRISPR-Cas inhibitors, represent a powerful, naturally occurring regulatory layer for CRISPR control. First …
Rapid characterization of anti-CRISPR proteins and ... - PubMed
Dec 11, 2023 · Anti-CRISPR (Acr) proteins are encoded by mobile genetic elements to overcome the CRISPR immunity of prokaryotes, displaying promises as controllable tools for modulating …
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