Learn how to phenotype LSK, LSK/SLAM, and ESLAM hematopoietic stem and progenitor cells from mouse bone marrow using flow cytometry.

Here we show that the Lin (-)Sca-1 (+)c-Kit (-) (LSK (-)) cell population derived from HSC-containing Lin (-)Sca-1 (+)c-Kit (+) (LSK) cells has significantly higher numbers of apoptotic cells. Depletion of LSK cells by radiation or the cytotoxic chemical 5-fluorouracil results in an expansion of the LSK (-) population.

The significance of a population in mouse bone marrow of lineage negative, Sca1 positive, c-kit negative (LSK-) cells, which is reported to be devoid of long-term repopulation capacity or myeloid potential, is unknown. Here, we show that the LSK- ...

筛选Lin-/SCA1+/c-KIT+细胞 (即LSK细胞)是分离小鼠HSC常用的方法。 但是，必须注意的是，不同品系的小鼠造血细胞Sca-1 表达量是不同的55。 例如，C57BL/6J小鼠Sca-1高表达，而其它品系小鼠，如BALB/c则表达量很低。 除利用Sca-1外，也可以用其它标志 或方法分离小鼠造血干/祖细胞。 如：依据细胞罗丹明123和Hoechst33342染料排出能力，或正筛选SLAM受体 （CD150），EPCR（CD201）或ESAM阳性细胞等56。 小鼠原始造血细胞也表达CD34，但 …

2017年9月12日 · Moreover, LSK cells, which include both HSCs and MPPs (together known as HSPCs), showed the highest expression among BM cells (Fig. 1c). In the myeloid lineage, LSK cells differentiate...

2019年6月4日 · We report that targeted disruption of Ash2l in the murine hematopoietic system results in the death of the mice due to a rapid loss of mature hematopoietic cells. However, lin − Sca1 + Kit +...

2021年6月10日 · Hematopoietic stem cells (HSCs) and distinct multipotent progenitor (MPP) populations (MPP1-4) contained within the Lin-Sca-1+c-Kit+ (LSK) compartment have previously been identified using diverse surface-marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+CD13 …

2012年6月4日 · In this study, we found that the LSK − population derived from HSC-containing LSK cells does not have stem cell functions but is capable of regulating the survival of the LSK population by providing a cellular path for irreversible transition of LSK to highly apoptotic LSK − …

LSK细胞就是小鼠骨髓中的造血干细胞。 从小鼠后退骨中提取出细胞，然后染色，上流式细胞仪，最后Gate出lsk细胞就认为是造血干细胞。

The best-characterized and most abundant MPP subset is defined as Flk2 + LSK cells and is now considered as a fully multipotent but lineage-biased population, with low megakaryocyte/erythroid (MegE) and high lymphoid potentials (Adolfsson et al., 2005; Forsberg et al., 2006; Boyer et al., 2011; Buza-Vidas et al., 2011).